TSKgel Amide-80, 2 µm

TSKgel Amide-80, 2 µm columns are packed with spherical silica particles that are covalently bonded with carbamoyl groups. The amide stationary phase provides a unique selectivity under regular normal phase conditions or in the hydrophilic interaction mode of chromatography.

TSKgel Amide-80, 2 µm columns possess superior stability in aqueous/organic solvent systems, an advantage over other hydrophilic stationary phases such as traditional amino-bonded phase columns. In addition, TSKgel Amide-80, 2 µm columns are ideally suited for sensitive LC/MS analysis of water-soluble polar compounds because the high organic content in the mobile phase provides rapid evaporation of solvent during electrospray ionization.

Target applications for the TSKgel Amide-80, 2 µm columns include the analysis of glycans and hydrophilic molecules.

The pore size of the bonded phase is indicated by the number in the product description, in this case TSKgel Amide-80 has 8 nm nominal pore size. The nominal pore size of the starting base silica is 10 nm.

P/N	Description	Particle Size (µm)	Housing Material	ID (mm)	Length (cm)	Price ($)
0023454	TSKgel Amide-80, 2 µm	2	Stainless Steel	2	5	1,104	Inquire
0023455	TSKgel Amide-80, 2 µm	2	Stainless Steel	2	10	1,158	Inquire
0023456	TSKgel Amide-80, 2 µm	2	Stainless Steel	2	15	1,217	Inquire
0023457	TSKgel Amide-80, 2 µm	2	Stainless Steel	3	5	1,158	Inquire
0023458	TSKgel Amide-80, 2 µm	2	Stainless Steel	3	10	1,217	Inquire
0023459	TSKgel Amide-80, 2 µm	2	Stainless Steel	3	15	1,276	Inquire

Product Attributes

Pore size (silica):	8 nm*
Particle size (mean):	2 µm
pH stability:	2.0-7.5
Functional group:	carbamoyl
Max. temperature (°C):	50
Surface area (m²/g)	450

*The pore size of the bonded phase is indicated by the number in the product description, in this case TSKgel Amide-80 has 8 nm nominal pore size. The nominal pore size of the starting base silica is 10 nm.

Structure:

Comparison of Glycan Peak Profile of Monoclonal Antibodies

Columns:	TSKgel Amide-80, 2 µm, 2.0 mm ID × 15 cm
Mobile phase:	A: 200 mmol/L acetic acid + triethylamine, pH 7.3 B: ACN
Gradient:	75% B (0-5 min) 75 - 50% (5 - 80 min, linear)
Flow rate:	0.5 mL/min
Detection:	fluorescence (λ_ex:315 nm,λ_em: 380 nm
Injection vol.:	50 µL
Samples:	A: pyridylaminated oligosaccharides released from mAb-1 (mouse) B: pyridylaminated oligosaccharides released from mAb-2 (human) C: PA-glucose oligomer (3 - 22 mer) (TaKaRa Bio)

Ultra-high Fast Separation with TSKgel
Amide-80, 2 µm Column

Column:	TSKgel Amide-80, 2 µm, 2.0 mm ID × 5 cm
Mobile phase:	20 mmol/L NH₄OAc, pH 4.7 / acetronitrile = 10/90
Flow rate:	1.29 mL/min
Detection:	UV @ 254 nm
Temperature:	40 °C
Injection vol.:	2 µL
Samples:	1. toluene (1 g/L) 2. theophylline (0.1 g/L) 3. theobromine (0.1 g/L) 4. NPβGlu (0.1 g/L) 5. NPαGlu (0.1 g/L) 6. 2'-deoxyuridine (0.1 g/L) 7. 5-methyluridine (0.1 g/L) 8. uridine (0.1 g/L)

For additional applications, please see our applications database.

Code #	Description	Literature Type
BR09	Solutions for Monoclonal Antibody Analysis and Characterization	Brochure
DS1052	TSKgel Amide-80 Products	Operating Conditions and Specifications
IM01	TSKgel Column Instruction Manual	Instruction Manual
LCAT04	TSKgel U/HPLC Columns 2019 Product Guide -- HILIC Columns	Product Catalog
PO42	TSKgel Amide-80, 2 µm Columns	Product Overview
SR055	Separation of Saccharides Using TSKgel Amide-80, a Packing Material for High-Performance Normal Phase Partition Chromatography (part 1)	Separation Report
SR079	Separation of Saccharides Using TSKgel Amide-80, a Packing Material for High Performance Normal Phase Partition Chromatography (part 2)	Separation Report

P/N	Description	Particle Size (µm)	Housing Material	ID (mm)	Length (cm)	Price ($)
0023460	TSKgel Amide-80 Guard Column for 2 mm ID, 2 µm columns	2	Stainless Steel	2	1	707