HPLC Columns

TSKgel G4000SW Glass

The TSKgel G4000SW Glass column is the glass format of the TSKgel G4000SW.  As the first generation of silica-based SEC columns, the TSKgel G4000SW column provides excellent separation of proteins and other biomolecules.

The TSKgel G4000SW Glass column is available in a 13 μm particle size analytical column.


P/NDescriptionParticle Size Housing MaterialID (mm)Length (cm)Price ($) 
08801TSKgel G4000SW Glass13Glass8301,651

Product Attributes

Base material: Silica
Particle size (mean): 13 µm
Pore size (mean): 45 nm
Functional group: Diol
pH stability: 2.5-7.5
Calibration range: 2.0 × 104- 1.0 × 10Da (globular proteins)



Calibration Curves for Globular Proteins, Polyethylene Oxides and Dextrans 
for TSKgel SW Columns


Columns: TSKgel SW columns, 7.5 mm ID × 60 cm × 2
Mobile phase: dextrans and polyethylene oxides: distilled water;
proteins: 0.3 mol/L NaCl in 0.1 mol/L phosphate buffer, pH7.0
Flow rate: 1.0 mL/min
Detection: UV @ 220 nm and RI
Temperature: A. 25°CB. 25°CC. 40°C
Samples: proteins (black circle),
polyethylene oxides (white circle),
dextrans (half circle)


Code #DescriptionLiterature Type
AO01 Gel Filtration Chromatography with TSKgel columns Application Overview
AO02 Purification of Nucleic Acids Application Overview
DS1007 TSKgel G4000SW ProductsOperating Conditions and Specifications
IM01 TSKgel Column Instruction ManualInstruction Manual
LCAT01A TSKgel U/HPLC Columns 2019 Product Guide -- SEC, SW Series ColumnsProduct Catalog
P/NDescriptionParticle Size Housing MaterialID (mm)Length (cm)Price ($) 
08805TSKgel G3000SW-G4000SW Guard Column for 8 mm ID glass columns10Glass84591