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HPLC Columns

TSKgel BioAssist DS

TSKgel BioAssist DS columns are designed for the desalting and buffer exchange of proteins and polynucleotides at semi-preparative scale. Packed with 15 μm polyacrylamide beads in PEEK hardware, TSKgel BioAssist DS columns show excellent desalting performance. These columns can be operated in standard HPLC systems to quickly and efficiently reduce salt and/or buffer concentrations of collected protein or nucleic acid fractions.

The novel* hydrophilic highly cross-linked polyacrylamide beads exhibit superior mechanical strength compared with conventional hydrophilic polyacrylamide beads and cross-linked dextran beads. This increase in strength is what allows the use of the small spherical 15 μm beads.

*US patent number 7,659,348

 

 
P/NDescriptionParticle Size Housing MaterialID (mm)Length (cm)Price ($) 
21464TSKgel BioAssist DS15PEEK1015751
21463TSKgel BioAssist DS15PEEK4.615472
         

Product Attributes

Base material: urea cross-linked polyacrylamide
Particle size (mean): 15 µm
Pore size (mean): excludes 2,500 Da PEG
Particle porosity: ca. 60%
Mechanical strength: <4 MPa

Polystyrene Calibration Curves

SEC_BioAssist-DS-Calibration-Fig23.png

Columns: Custom TSKgel BioAssist DS column, 6 mm ID × 15 cm
Mobile phase: distilled H2O
Flow rate: 0.5 mL/min
Sample: ethylene glycol, PEGs

       

Desalting of Proteins

 

SEC_BioAssist-DS-Fig24.png

Columns: TSKgel BioAssist DS, 15 µm,
10 mm ID × 15 cm
Mobile phase: 0.1 mol/L KH2PO/K2HPO4, pH 6.7, 0.1 mol/L Na2SO4 + 0.005% NaN3
Proteins in 0.1 mol/L phosphate buffer, pH 6.7
Flow rate: 0.8 mL/min (4.6 mm ID) and 1.0 mL/min (10 mm ID)
Detection: UV @ 80 nm and RI
Temperature: 25 °C
Injection vol.: 10 µL

For additional applications, please see our applications database.

 
Code #DescriptionLiterature Type
AN030 A Novel Desalting Column With High Mechanical Strength for Faster Desalting of ProteinsApplication Note
AO01 Gel Filtration Chromatography with TSKgel columns Application Overview
DS1237 TSKgel BioAssist DS ProductsOperating Conditions and Specifications
IM01 TSKgel Column Instruction ManualInstruction Manual
LCAT01A TSKgel U/HPLC Columns 2019 Product Guide -- SEC, SW Series ColumnsProduct Catalog
TP159 Characteristics of a Desalting Column for Biomolecular Analysis: the TSKgel BioAssist DS ColumnTechnical Presentation
TP160 A Novel Desalting Column with High Mechanical Strength for Faster Desalting of Proteins Technical Presentation
TP167 Fast Desalting of Proteins Using a Novel High Mechanical Strength Gel Filtration ColumnTechnical Presentation