Products

HPLC Columns

TSKgel SuperSW mAb HR

The TSKgel SuperSW mAb HR column offers high resolution analysis of mAb fragments, monomer, and dimer. The high resolution capability of this column is due to a unique pore-controlled technology, which produces a shallow calibration curve in the molecular weight region of a typical monoclonal antibody.

 

 
P/NDescriptionParticle Size Housing MaterialID (mm)Length (cm)Price ($) 
22854TSKgel SuperSW mAb HR4Stainless Steel7.8301,857
          

Product Attributes

Base material: Silica
Particle size (mean): 4 µm
Pore size (mean): 25 nm
Functional group: Diol
pH stability: 2.5-7.5
Calibration range: 1 × 104 - 5 × 105 Da (globular proteins)

Protein Calibration Curves

SEC_SuperSW-mAb-HR-Calibration-New-Fig1.png


                   

Comparison of Resolution of mAb Monomer and Dimer

 

SEC_SuperSW-mAb-HR-New-Fig2rev.png

 

Columns: A. TSKgel SuperSW mAb HR, 4 µm, 7.8 mm ID × 30 cm
B. Brand A, 3 µm,
7.8 mm ID × 30 cm
C. Brand B, 5 µm,
7.8 mm ID × 30 cm
D. Brand C, 5 µm,
8.0 mm ID × 30 cm
E. Brand D, 5 µm
8.0 mm ID  30 cm
Mobile phase: 200 mmol/L phosphate buffer, pH 6.7 + 0.05% NaN3
Flow rate: 1.0 mL/min
Detection: UV @ 280 nm
Temperature: 25 °C
Injection vol.: 10 µL
Samples: lgG (human polyclonal), 1.0 g/L

High Durability of TSKgel SuperSW mAb HR Column

 

SEC_SuperSW-mAb-HR-New-Fig3.png

 

Column: TSKgel SuperSW mAb HR, 4 µm,
7.8 mm ID × 30 cm

Mobile phase: 0.2 mol/L phosphate buffer, pH 6.7 + 0.05% NaN3
Flow rate: 0.8 mL/min
Detection: UV @ 280 nm
Injection vol.: 10 µL
Samples: 1. γ-Globulin
2. Cytochrome C
3. DNP-L-Alanine

 

Code #DescriptionLiterature Type
AN051 Separation of a Monoclonal Antibody Monomer from its Impurities using New TSKgel SW mAb ColumnsApplication Note
AN058 Separation of Monoclonal Antibody (mAb) Monomer from its Half-body using Size Exclusion ChromatographyApplication Note
DS1239 TSKgel SW mAb ProductsOperating Conditions and Specifications
IM01 TSKgel Column Instruction ManualInstruction Manual
LCAT01A TSKgel U/HPLC Columns 2019 Product Guide -- SEC, SW Series ColumnsProduct Catalog
PO35 TSKgel SuperSW mAb HR, TSKgel SuperSW mAb HTP and TSKgel UltraSW Aggregate ColumnsProduct Overview
TP166 New silica-based SEC columns designed for the separation of mAb monomers and their impuritiesTechnical Presentation
TP175 Three novel prototype SEC columns for the separation of an antibody monomer from its dimer, higher aggregates, and antibody fragmentsTechnical Presentation
TP180 Three novel prototype silica-based SEC columns designed for the separation of an antibody monomer from its dimer, higher aggregates, and antibody fragmentsTechnical Presentation
TP181 Detection of protein heterogeneity by HPLC (additional data included)Technical Presentation
TP183 High Resolution Analysis of Monoclonal Antibody Heterogeneity by Size Exclusion Chromatography and Hydrophilic Interaction ChromatographyTechnical Presentation
TP185 Three Novel Size Exclusion Chromatography Columns Designed for the Separation of a Monoclonal Antibody Monomer from its Impurities Technical Presentation
TP195 Separation of Half-mAb and Half-mAb Equivalents with High Resolution using Size Exclusion Chromatography Packed with a Unique Controlled Pore Technology Technical Presentation
TP197 Evaluation of Mobile Phase Conditions on the Resolution of Monoclonal Antibody Monomer from its Protein Variants Separated on a TSKgel SuperSW mAb HR SEC ColumnTechnical Presentation
P/NDescriptionParticle Size Housing MaterialID (mm)Length (cm)Price ($) 
22857TSKgel SuperSW mAb HR Guard Column for 7.8 mm ID column4Stainless Steel64673