Products

HPLC Columns

TSKgel UltraSW Aggregate

The TSKgel UltraSW Aggregate column is designed with a higher exclusion limit and smaller particle size (3 µm) than the other TSKgel SW mAb columns and is for the high resolution analysis of higher order mAb aggregates.

 

The larger molecular weight exclusion limit of the TSKgel UltraSW Aggregate column allows for superior resolving power for multimers and aggregates of large proteins, including thyroglobulin and IgG.

 

 
P/NDescriptionParticle Size Housing MaterialID (mm)Length (cm)Price ($) 
22856TSKgel UltraSW Aggregate3Stainless Steel7.8301,946
           

Product Attributes

Base material: Silica
Particle size (mean): 3 µm
Pore size (mean): 30 nm
Functional group: Diol
pH stability: 2.5-7.5
Calibration range: 1 × 104- 2 × 10Da
(globular proteins)

Protein Calibration Curves

SEC_SuperSW-mAb-HR-Calibration-New-Fig1.png


               

Separation of Forced Aggregated mAb 1 Using Volatile and Salt-based Mobile Phase Compositions 

 

SEC_UltraSW-Aggregate-AN68_Fig2rev.png

Columns: TSKgel UltraSW Aggregate, 3μm,
7.8 mm ID × 30 cm
Mobile phase: 100 mmol/L PO4/100 mmol/L SO4,
pH 6.7
20% CH3CN/0.1% TFA/0.1% FA 
100 mmol/L ammonium bicarbonate,
pH 7.0
Gradient: isocratic
Flow rate: 1 mL/min
Detection: UV @ 280 nm
Temperature: 25 °C
Injection vol.: 10 μL
Sample: TBL mAb 1 (4.0 g/L)

 

 

Separation of mAb Trimer and Dimer

SEC_UltraSW-Aggregate-New-Fig6rev.png

Column: TSKgel UltraSW Aggregate, 3 μm,
7.8 mm ID × 30 cm

Mobile phase: 0.2 mol/L phosphate buffer, pH 6.7 + 0.05% NaN3
Flow Rate: 0.8 mL/min
Detection: UV @ 280 nm
Temperature: 25 °C
Sample: monoclonal antibody-2
(mouse-human chimeric IgG, Erbitux), 10 μL

 

Analysis of Heat Induced Forced Denatured, Large Hydrophobic Metalloprotein, Apoferritin

 

SEC_UltraSW-Aggregate-New-Fig7rev.png

 

Columns: TSKgel UltraSW Aggregate, 3 μm,
7.8 mm ID × 30 cm

Mobile phase: 50 mmol/L potassium phosphate (monobasic),
50 mmol/L sodium phosphate (dibasic),
100 mmol/L sodium sulfate, 0.05% NaN3, pH 6.7
Flow rate: 1.0 mL/min
Detection: UV @ 280 nm
Temperature: 30 °C
Injection vol.: 10 μL
Samples: ferritin - Sigma, 4.7 g/L, in saline (0.9% NaCl in water) solution, stored at 2-8°C
apoferritin - Sigma, 5.0 g/L, in 50% glycerol and 0.075 mol/L sodium chloride, stored at -20°C


For additional applications, please see our applications database.

 

Code #DescriptionLiterature Type
AN051 Separation of a Monoclonal Antibody Monomer from its Impurities using New TSKgel SW mAb ColumnsApplication Note
AN053 Analysis of Monoclonal Antibody and Protein Aggregates Induced by Denaturation using a Novel Size Exclusion Chromatography ColumnApplication Note
AN060 Improving Recovery and Resolution of mAb Aggregates using Mobile Phase Additives in SEC Application Note
AN068 Analysis of Monoclonal Antibody Aggregates by SEC using MS-Friendly Mobile PhasesApplication Note
AN081 Monitoring Protein Unfolding and RefoldingApplication Note
DS1239 TSKgel SW mAb ProductsOperating Conditions and Specifications
IM01 TSKgel Column Instruction ManualInstruction Manual
LCAT01A TSKgel U/HPLC Columns 2019 Product Guide -- SEC, SW Series ColumnsProduct Catalog
PO35 TSKgel SuperSW mAb HR, TSKgel SuperSW mAb HTP and TSKgel UltraSW Aggregate ColumnsProduct Overview
TP166 New silica-based SEC columns designed for the separation of mAb monomers and their impuritiesTechnical Presentation
TP175 Three novel prototype SEC columns for the separation of an antibody monomer from its dimer, higher aggregates, and antibody fragmentsTechnical Presentation
TP180 Three novel prototype silica-based SEC columns designed for the separation of an antibody monomer from its dimer, higher aggregates, and antibody fragmentsTechnical Presentation
TP181 Detection of protein heterogeneity by HPLC (additional data included)Technical Presentation
TP185 Three Novel Size Exclusion Chromatography Columns Designed for the Separation of a Monoclonal Antibody Monomer from its Impurities Technical Presentation
TP187 Separation of Proteins and Monoclonal Antibodies using New Wide Pore C4 Phase Silica Gel Reversed Phase Column with Moderate Hydrophobicity Designed for Protein SeparationTechnical Presentation
TP188 Separation of Proteins with a Wide Variety of Hydrophobicity using a Reversed Phase 30 nm Pore Size Short Alkyl Chain ColumnTechnical Presentation
TP190 Characterization of Two Novel Analytical Chromatographic Columns for Orthogonal Analysis of Monoclonal Antibodies and Protein Aggregates and Their IsoformsTechnical Presentation
TP193 Separation of Monoclonal Antibody and Protein Aggregates Induced by Thermal Denaturation, using a Novel Size Exclusion Chromatography Column Technical Presentation
TP194 Stability Studies of a Novel Silica-Based, Diol-Bonded Size Exclusion Chromatography Analytical Column Specifically Designed for the Separation of Aggregates Technical Presentation
TP206 Use of 3 μm Analytical Size Exclusion Chromatography (SEC) Column for Monitoring Protein Unfolding and Refolding in Stability Studies of Proteins and Monoclonal AntibodiesTechnical Presentation
TP211 Effect of Larger Exclusion Limit on the Separation of HMW Species – a Study Using a 3 μm Size Exclusion Chromatography ColumnTechnical Presentation
P/NDescriptionParticle Size Housing MaterialID (mm)Length (cm)Price ($) 
22859TSKgel UltraSW Aggregate Guard Column for 7.8 mm ID column3Stainless Steel64716