TSKgel G2000SWxl

Widely considered the premium columns for analyzing biopolymers by GFC, TSKgel G2000SWxl, 5 μm columns are an excellent choice for small proteins and peptide separations. The fractionation range is applicable for globular protein samples up to 150,000 Da. These columns feature high pore volume per unit column volume, low sample adsorption and excellent column efficiency, all contributing to unsurpassed sample resolution.

Also available within the TSKgel G2000SWxl column line is a BioAssist column made of PEEK housing material to reduce sample adsorption to stainless steel or glass, and QC-PAK columns, which are 15 cm in length with 5 µm particles and offer the same resolution in half the time as the 30 cm TSKgel G2000SWxl column.

P/N	Description	Particle Size (µm)	Housing Material	ID (mm)	Length (cm)	Price ($)
0008540	TSKgel G2000SWxl	5	Stainless Steel	7.8	30	2,219	Inquire

Product Attributes

Base material:	Silica
Particle size (mean):	5 µm
Pore size (mean):	12.5 nm
Functional group:	Diol
pH stability:	2.5-7.5
Calibration range:	5,000 - 1.5 × 10⁵Da (globular proteins)

Calibration Curves

Column:	TSKgel SWXL columns, 7.8 mm ID × 30 cm
Mobile phase:	0.3 mol/L NaCl in 0.1 mol/L sodium phosphate buffer, pH 7.0
Detection:	UV @ 220 nm
Samples:	1. thyroglobulin (6.6 × 10⁵Da) 2. IgG (1.56 × 10⁵Da) 3. bovine serum albumin (6.7 × 10⁴Da) 4. ovalbumin (4.3 × 10⁴Da) 5. peroxidase(4.02 × 10⁴Da) 6. β-lactoglobulin (3.5 × 10⁴Da) 7. myoglobin (1.69 × 10⁴Da) 8. ribonuclease A (1.37 × 10⁴Da) 9. cytochrome C (1.24 × 10⁴Da) 10. glycine tetramer (246 Da)

Separation of Crude Extract of Rat Liver (10 µL)

Columns:	TSKgel G2000SWXL, 5 µm, 7.8mm ID × 30 cm
Mobile phase:	0.05 mol/L phosphate buffer, pH 7.0, + 0.3 mol/L NaCl
Flow rate:	1 mL/min
Detection:	UV @ 220 nm
Temperature.:	25 °C

Separation of Reduced Monoclonal Antibody in the Separation of BSA

Column:	TSKgel G2000SWXL, 5 µm, 7.8 mm ID × 30 cm
Mobile phase:	100 mmol/L potassium phosphate buffer, 100 mmol/L sodium sulfate, pH 6.7 + 0.05 NaN₃
Flow rate:	0.2 mL/min
Detection:	UV @ 280 nm
Temperature:	ambient, 37 °C
Injection vol.:	10 µL
Sample:	BSA

For additional applications, please see our applications database.

Code #	Description	Literature Type
AN046	UHPLC Separations using HPLC Methods and TSKgel Columns	Application Note
AO01	Gel Filtration Chromatography with TSKgel columns	Application Overview
AO02	Purification of Nucleic Acids	Application Overview
DS1008	TSKgel G2000SWxl Products	Operating Conditions and Specifications
IM01	TSKgel Column Instruction Manual	Instruction Manual
LCAT01A	TSKgel U/HPLC Columns 2019 Product Guide -- SEC, SW Series Columns	Product Catalog
SR046	TSKgel SWxl Series Columns	Separation Report
SR074	Separation of Monoclonal Antibodies Using TSKgel HPLC Columns	Separation Report

P/N	Description	Particle Size (µm)	Housing Material	ID (mm)	Length (cm)	Price ($)
0008543	TSKgel G2000SWxl-G4000SWxl, and QC-PAK GFC Guard Column for 7.8 mm ID columns	7	Stainless Steel	6	4	792