Products

HPLC Columns

TSKgel G2000SWxl

Widely considered the premium columns for analyzing biopolymers by GFC, TSKgel G2000SWxl, 5 μm columns are an excellent choice for small proteins and peptide separations. The fractionation range is applicable for globular protein samples up to 150,000 Da. These columns feature high pore volume per unit column volume, low sample adsorption and excellent column efficiency, all contributing to unsurpassed sample resolution.

Also available within the TSKgel G2000SWxl column line is a BioAssist column made of PEEK housing material to reduce sample adsorption to stainless steel or glass, and QC-PAK columns, which are 15 cm in length with 5 µm particles and offer the same resolution in half the time as the 30 cm TSKgel G2000SWxl column.

 

 
P/NDescriptionParticle Size Housing MaterialID (mm)Length (cm)Price ($) 
08540TSKgel G2000SWxl5Stainless Steel7.8301,775
           

Product Attributes

Base material: Silica
Particle size (mean): 5 µm
Pore size (mean): 12.5 nm
Functional group: Diol
pH stability: 2.5-7.5
Calibration range: 5,000 - 1.5 × 10Da
(globular proteins)

Calibration Curves

SEC_G2000SWxl-Calibration-rev.png

Column: TSKgel SWXL  columns,
7.8 mm ID × 30 cm
Mobile phase: 0.3 mol/L NaCl in 0.1 mol/L sodium phosphate buffer, pH 7.0
Detection: UV @ 220 nm
Samples: 1. thyroglobulin (6.6 × 10Da)
2. IgG (1.56 × 10Da)
3. bovine serum albumin
(6.7 × 10Da)
4. ovalbumin (4.3 × 10Da)
5. peroxidase(4.02 × 10Da)
6. β-lactoglobulin (3.5 × 10Da)
7. myoglobin (1.69 × 10Da)
8. ribonuclease A (1.37 × 10Da)
9. cytochrome C (1.24 × 10Da)
10. glycine tetramer (246 Da)

                

Separation of Crude Extract of Rat Liver (10 µL)

SEC_G2000SWxl-Fig11rev.png

 

Columns: TSKgel G2000SWXL, 5 µm,
7.8mm ID × 30 cm

Mobile phase: 0.05 mol/L phosphate buffer, pH 7.0,
+ 0.3 mol/L NaCl
Flow rate: 1 mL/min
Detection: UV @ 220 nm
Temperature.: 25 °C 

 

Separation of Reduced Monoclonal Antibody in the Separation of BSA

 

SEC_G2000SWxl_Slide15rev.png

 

Column: TSKgel G2000SWXL, 5 µm,
7.8 mm ID × 30 cm
Mobile phase: 100 mmol/L potassium phosphate buffer, 100 mmol/L sodium sulfate, pH 6.7 + 0.05 NaN3
Flow rate: 0.2 mL/min
Detection: UV @ 280 nm
Temperature: ambient, 37 °C
Injection vol.: 10 µL
Sample: BSA

For additional applications, please see our applications database.

 

Code #DescriptionLiterature Type
AN046 UHPLC Separations using HPLC Methods and TSKgel ColumnsApplication Note
AO01 Gel Filtration Chromatography with TSKgel columns Application Overview
AO02 Purification of Nucleic Acids Application Overview
DS1008 TSKgel G2000SWxl ProductsOperating Conditions and Specifications
IM01 TSKgel Column Instruction ManualInstruction Manual
IM20 Regeneration of TSKgel® SEC-SW HPLC ColumnsInstruction Manual
LCAT01A TSKgel U/HPLC Columns 2019 Product Guide -- SEC, SW Series ColumnsProduct Catalog
SR046 TSKgel SWxl Series ColumnsSeparation Report
SR074 Separation of Monoclonal Antibodies Using TSKgel HPLC ColumnsSeparation Report
P/NDescriptionParticle Size Housing MaterialID (mm)Length (cm)Price ($) 
08543TSKgel G2000SWxl-G4000SWxl, and QC-PAK GFC Guard Column for 7.8 mm ID columns7Stainless Steel64633