HPLC Columns

TSKgel G3000PWxl-CP

The TSKgel G3000PWxl-CP column was specifically developed for the analysis of water-soluble cationic polymers with molecular weights up to 100,000 Da. Composed of 7 µm polymethacrylate beads, cationic groups are introduced on the surface of the TSKgel G3000PWxl-CP packing material to prevent adsorption of cationic polymers and allow elution under low salt conditions.

This columns shows high theoretical plate numbers, linear calibration curves and high durability because the base resin is the same as that used in the TSKgel PWxl series columns.


P/NDescriptionParticle Size Housing MaterialID (mm)Length (cm)Price ($) 
0021873TSKgel G3000PWxl-CP7Stainless Steel7.8302,432

Product Attributes

Base material: polymethacrylate
Particle size (mean): 7 µm
Pore size (mean): 20 nm
Exclusion limit: 1.0 × 105 Da
Separation range (PEO,PEG) 200 ~ 5.0 × 104 Da
Theoretical plates: 16,000

PEO and PEG Calibration Curves


Columns: TSKgel G3000WXL-CP, 7 µm,
7.8 mm ID × 30 cm
TSKgel G5000WXL-CP, 10 µm,
7.8 mm ID × 30 cm
TSKgel G6000WXL-CP, 13 µm,
7.8 mm ID × 30 cm

Mobile phase: H2O with 0.1 mol/L NaNO3
Flow rate: 1.0 mL/min
Detection: Rl
Temperature: 25 °C
Samples: polyethylene oxides (PEO) standards
polyethylene glycols (PEG) standards


Analysis of Cationic Polymers



Columns: TSKgel G3000PWXL-CP, 7 μm,
7.8 mm ID × 30 cm 
TSKgel G5000PWXL-CP, 10 μm, 
7.8 mm ID × 30 cm
TSKgel G6000PWXL-CP, 13 μm, 
7.8 mm ID × 30 cm

Mobile phase: H2O with 0.1 mol/L NaNO3
Flow rate: 1 mL/min
Detection: RI
Temperature: 25 °C
Sample Load: 3 g/L, 100 μL


Elution Profiles of PAA and PEI Polymers



Column: TSKgel G3000WXL-CP, 7 μm,
7.8 mm ID × 30 cm × 2

Mobile phase: H2O with 0.1 mol/L NaNO3
Flow rate: 1.0 mL/min
Detection: RI
Temperature: 25 °C
Sample: polyethyleneimine (PEI)
polyallylamine-HCl (PAA)

For additional applications, please see our applications database.


Code #DescriptionLiterature Type
AN016 TSKgel PWxl-CP Size Exclusion Chromatography for the Analysis of Water-Soluble Cationic PolymersApplication Note
AO01 Gel Filtration Chromatography with TSKgel columns Application Overview
DS1227 TSKgel PWxl-CP ProductsOperating Conditions and Specifications
IM01 TSKgel Column Instruction ManualInstruction Manual
LCAT01B TSKgel U/HPLC Columns 2019 Product Guide -- SEC, PW Series ColumnsProduct Catalog
PO17 TSKgel PWXL-CP Columns Size Exclusion Chromatography for Cationic Polymer AnalysisProduct Overview
SR106 Aqueous SEC Columns for Analysis of Cationic Polymers: TSKgel PWxl-CP SeriesSeparation Report
P/NDescriptionParticle Size Housing MaterialID (mm)Length (cm)Price ($) 
0021876TSKgel G3000-G6000PWxl-CP Guard Column for 7.8 mm ID columns 13Stainless Steel64815