Analysis of Membrane Protein with Differing Surfactant Concentrations in the Mobile Phase
| Columns: |
TSKgel G3000SW, 10 μm,
7.5 mm ID × 60 cm |
| Mobile phase: |
(0.2 mol/L sodium choloride + 20% glycerol + octaethylene glycol dodecylether) in 50 mmol/L phosphate buffer, pH 7.0
Note: concentration of surfactant:
1. 0.005% 2. 0.01% 3. 0.025%
4. 0.05% |
| Flow rate: |
1.0 mL/min
|
| Detection: |
UV @ 280 nm |
| Sample: |
membrane protein from a crude extract from a rat liver microsome |
Analysis of Metallothionein
| Columns: |
TSKgel G3000SW, 13 μm,
21.5 mm ID × 60 cm |
| Mobile phase: |
50 mmol/L Tris-HCL buffer |
| Detection: |
atomic absorption (Cd, Zn) + UV @ 280 nm
A: Cd
B: Zn
|
| Samples*: |
rat liver supernatant
1.metallothionein I
2.metallothionein II
|
|
|
Tracking Changes Over Time
| Column: |
TSKgel G3000SW, 10 μm,
7.5 mm ID × 60 cm
|
| Mobile phase: |
0.1 mol/L acetate buffer, pH 5.0 + 0.1 mol/L sodium sulfate |
| Sample: |
100 μL solutions produced by digestion of lgG (20 g/L) by pepsin after 0, 2, 4, 6, 8, 10, 15, 30 and 60 minutes |
|
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