Products

HPLC Columns

TSKgel G3000SW

As the first generation of silica-based GFC columns, TSKgel G3000SW columns provide excellent separation of proteins and other biomolecules with molecular weights up to 500,000 Da.

The TSKgel G3000SW columns are available in 10 and 13 μm for analytical and semi-preparative purifications.

 

 
P/NDescriptionParticle Size Housing MaterialID (mm)Length (cm)Price ($) 
05789TSKgel G3000SW10Stainless Steel7.5301,439
05103TSKgel G3000SW10Stainless Steel7.5602,354
06728TSKgel G3000SW13Stainless Steel21.5304,003
05147TSKgel G3000SW13Stainless Steel21.5605,819
           

Product Attributes

Base material: Silica
Particle size (mean): 10 µm, 13 µm
Pore size (mean): 25 nm
Functional group: Diol
pH stability: 2.5-7.5
Calibration range: 1.0 × 104 - 1.0 × 10Da (globular proteins)

          

 

Calibration Curves for Globular Proteins, Polyethylene Oxides and Dextrans 
for TSKgel SW Columns

SEC_G2000SW-Calibration-curves-rev.png

Columns: TSKgel SW columns, 7.5 mm ID × 60 cm × 2
Mobile phase: dextrans and polyethylene oxides: distilled water;
proteins: 0.3 mol/L NaCl in 0.1 mol/L phosphate buffer, pH7.0
Flow rate: 1.0 mL/min
Detection: UV @ 220 nm and RI
Temperature: A. 25°CB. 25°CC. 40°C
Samples: proteins (black circle),
polyethylene oxides (white circle),
dextrans (half circle)

 

              

Analysis of Membrane Protein with Differing Surfactant Concentrations in the Mobile Phase

SEC_G3000SW-Fig4.png


Columns: TSKgel G3000SW, 10 μm,
7.5 mm ID × 60 cm
Mobile phase: (0.2 mol/L sodium choloride + 20% glycerol + octaethylene glycol dodecylether) in 50 mmol/L phosphate buffer, pH 7.0
Note: concentration of surfactant:
1. 0.005% 2. 0.01% 3. 0.025%
4. 0.05%
Flow rate: 1.0 mL/min
Detection: UV @ 280 nm
Sample: membrane protein from a crude extract from a rat liver microsome

 

Analysis of Metallothionein

SEC_G3000-Fig7.png

Columns: TSKgel G3000SW, 13 μm,
21.5 mm ID × 60 cm
Mobile phase: 50 mmol/L Tris-HCL buffer
Detection: atomic absorption (Cd, Zn) + UV @ 280 nm
A: Cd
B: Zn
Samples*: rat liver supernatant
1.metallothionein I
2.metallothionein II

Tracking Changes Over Time

SEC_G3000SW-Fig5.png

Column: TSKgel G3000SW, 10 μm,
7.5 mm ID × 60 cm

Mobile phase: 0.1 mol/L acetate buffer, pH 5.0 + 0.1 mol/L sodium sulfate
Sample: 100 μL solutions produced by digestion of lgG (20 g/L) by pepsin after 0, 2, 4, 6, 8, 10, 15, 30 and 60 minutes

For additional applications, please see our applications database.

 

Code #DescriptionLiterature Type
AO01 Gel Filtration Chromatography with TSKgel columns Application Overview
AO02 Purification of Nucleic Acids Application Overview
DS1006 TSKgel G3000SW ProductsOperating Conditions and Specifications
DS1069 Preparative TSKgel G3000SW ColumnsOperating Conditions and Specifications
IM01 TSKgel Column Instruction ManualInstruction Manual
LCAT01A TSKgel HPLC Columns 2016 Product Guide -- SEC, SW Series ColumnsProduct Catalog
SR024 Applications for High-Performance Gel Filtration Columns (TSKgel SW and TSKgel PW columns): Focus on BiopolymersSeparation Report
P/NDescriptionParticle Size Housing MaterialID (mm)Length (cm)Price ($) 
05371TSKgel G2000SW-G4000SW Guard Column for 7.5 mm ID columns10Stainless Steel7.57.5540
05758TSKgel G2000SW-G4000SW Guard Column for 21.5 mm ID columns13Stainless Steel21.57.51212