Products

HPLC Columns

TSKgel SuperSW2000

Featuring  4 μm particles, the TSKgel SuperSW2000 column provides high efficiency for peptides and proteins. The 12.5 nm pore size results in a fractionation range up to 150,000 Da for globular proteins.  The narrow 4.6 mm inner diameter of the TSKgel SuperSW2000 column provides higher sensitivity in sample-limited cases and to cut down on solvent use. It is important to employ an HPLC system that is optimized with regards to extra-column band broadening to take full advantage of the high column efficiency that can be obtained on TSKgel SuperSW columns.

 

 
P/NDescriptionParticle Size Housing MaterialID (mm)Length (cm)Price ($) 
18674TSKgel SuperSW20004Stainless Steel4.6301,816
         

Product Attributes

Base material: silica
Particle size (mean): 4 µm
Pore size (mean): 12.5 nm
Functional group: Diol
pH stability: 2.5-7.5
Calibration range: 5,000 - 1.5 × 105 Da
(globular proteins)


Protein Calibration Curves

SEC_SuperSW2000-ProteinsCalibration_rev.png              

Columns: TSKgel SuperSW columns,
4.6 mm ID × 30 cm
Mobile phase: 0.2 mol/L phosphate buffer, pH 6.7
Flow rate: 0.35 mL/min
Detection: UV @ 280 nm
Samples: standard proteins
(5 µL, 0.1 g/L each)
1. thyroglobulin
2. γ-globulin
3. bovine serum albumin
4. β-lactoglobulin
5. lysozyme
6. cytochrome C
7. glycine tetramer

PEO and PEG Calibration Curves

SEC_SuperSW2000-PEO-PEGCalibration_rev.png

Columns: TSKgel SuperSW columns,
4.6 mm ID × 30 cm
Mobile phase: 0.05% sodium azide aqueous solution
Flow rate: 0.35 mL/min
Detection: RI
Temperature: 25 °C
Samples: polyethylene oxides (PEO) standards
polyethylene glycols (PEG) standards
(5 µL)

 

        

Analysis of Peptides

 

SEC_SuperSW2000-Fig22.png


 

Columns: TSKgel SuperSW2000, 4 µm,
4.6 mm ID × 30 cm
Mobile phase: 0.1% TFA in 45% aq. ACN
Flow rate: 0.35 mL/min
Detection: UV @ 210 nm
Injection vol.: 3 µL
Samples: Peptide P12: Tyr-Gly-Gly-Phe-Met-Arg-Gly-Leu
Peptide P16: Trp-Gly-Gly-Tyr
Peptide P19: Gly-Trp-Gly
Peptide P20: H-Ser-Tyr-Ser-Met-Glu-His-Phe-Arg-Trp-Gly-Lys-Pro-Val-Gly-Lys-Gly-Lys-Arg-Arg-Pro-Val-Lys-Val-Tyr-Pro-OH
Peptide P22: H-Ala-Gly-Cys-Lys-Asn-Phe-Phe-Trp-Lys-Thr-Phe-Thr-Ser-Cys-OH

For additional applications, please see our applications database.

 

Code #DescriptionLiterature Type
AO01 Gel Filtration Chromatography with TSKgel columns Application Overview
DS1156 TSKgel SuperSW2000 ProductsOperating Conditions and Specifications
IM01 TSKgel Column Instruction ManualInstruction Manual
LCAT01A TSKgel U/HPLC Columns 2019 Product Guide -- SEC, SW Series ColumnsProduct Catalog
PO06 TSKgel SuperSW2000 and SuperSW3000 columnsProduct Overview
SR095 TSKgel SuperSW SeriesSeparation Report
P/NDescriptionParticle Size Housing MaterialID (mm)Length (cm)Price ($) 
18762TSKgel SuperSW2000 and SuperSW3000 Guard Column for 4.6 mm ID columns4Stainless Steel4.63.5639