TSKgel SuperSW2000

Featuring 4 μm particles, the TSKgel SuperSW2000 column provides high efficiency for peptides and proteins. The 12.5 nm pore size results in a fractionation range up to 150,000 Da for globular proteins. The narrow 4.6 mm inner diameter of the TSKgel SuperSW2000 column provides higher sensitivity in sample-limited cases and to cut down on solvent use. It is important to employ an HPLC system that is optimized with regards to extra-column band broadening to take full advantage of the high column efficiency that can be obtained on TSKgel SuperSW columns.

P/N	Description	Particle Size (µm)	Housing Material	ID (mm)	Length (cm)	Price ($)
0018674	TSKgel SuperSW2000	4	Stainless Steel	4.6	30	2,270	Inquire

Product Attributes

Base material:	silica
Particle size (mean):	4 µm
Pore size (mean):	12.5 nm
Functional group:	Diol
pH stability:	2.5-7.5
Calibration range:	5,000 - 1.5 × 10⁵ Da (globular proteins)

Protein Calibration Curves

Columns:	TSKgel SuperSW columns, 4.6 mm ID × 30 cm
Mobile phase:	0.2 mol/L phosphate buffer, pH 6.7
Flow rate:	0.35 mL/min
Detection:	UV @ 280 nm
Samples:	standard proteins (5 µL, 0.1 g/L each) 1. thyroglobulin 2. γ-globulin 3. bovine serum albumin 4. β-lactoglobulin 5. lysozyme 6. cytochrome C 7. glycine tetramer

PEO and PEG Calibration Curves

Columns:	TSKgel SuperSW columns, 4.6 mm ID × 30 cm
Mobile phase:	0.05% sodium azide aqueous solution
Flow rate:	0.35 mL/min
Detection:	RI
Temperature:	25 °C
Samples:	polyethylene oxides (PEO) standards polyethylene glycols (PEG) standards (5 µL)

Analysis of Peptides

Columns:	TSKgel SuperSW2000, 4 µm, 4.6 mm ID × 30 cm
Mobile phase:	0.1% TFA in 45% aq. ACN
Flow rate:	0.35 mL/min
Detection:	UV @ 210 nm
Injection vol.:	3 µL
Samples:	Peptide P12: Tyr-Gly-Gly-Phe-Met-Arg-Gly-Leu Peptide P16: Trp-Gly-Gly-Tyr Peptide P19: Gly-Trp-Gly Peptide P20: H-Ser-Tyr-Ser-Met-Glu-His-Phe-Arg-Trp-Gly-Lys-Pro-Val-Gly-Lys-Gly-Lys-Arg-Arg-Pro-Val-Lys-Val-Tyr-Pro-OH Peptide P22: H-Ala-Gly-Cys-Lys-Asn-Phe-Phe-Trp-Lys-Thr-Phe-Thr-Ser-Cys-OH

For additional applications, please see our applications database.

Code #	Description	Literature Type
AO01	Gel Filtration Chromatography with TSKgel columns	Application Overview
DS1156	TSKgel SuperSW2000 Products	Operating Conditions and Specifications
IM01	TSKgel Column Instruction Manual	Instruction Manual
LCAT01A	TSKgel U/HPLC Columns 2019 Product Guide -- SEC, SW Series Columns	Product Catalog
PO06	TSKgel SuperSW2000 and SuperSW3000 columns	Product Overview
SR095	TSKgel SuperSW Series	Separation Report

P/N	Description	Particle Size (µm)	Housing Material	ID (mm)	Length (cm)	Price ($)
0018762	TSKgel SuperSW2000 and SuperSW3000 Guard Column for 4.6 mm ID columns	4	Stainless Steel	4.6	3.5	800