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HPLC Columns

TSKgel SuperSW3000

TSKgel SuperSW3000 are packed with ultra-efficient 4 μm particles,  providing  high efficiency separations. The 25 nm pore size results in a fractionation range up to 500,000 Da for globular proteins. The narrow 4.6 mm column inner diameter reduces buffer consumption and is ideal for sample-limited applications. 

It is important to employ an HPLC system that is optimized with regards to extra-column band broadening to take full advantage of the high column efficiency that can be obtained on TSKgel SuperSW columns.

 

 
P/NDescriptionParticle Size Housing MaterialID (mm)Length (cm)Price ($) 
21845TSKgel SuperSW30004Stainless Steel1302,084
21485TSKgel SuperSW30004Stainless Steel2302,084
18675TSKgel SuperSW30004Stainless Steel4.6301,816
          

Product Attributes

Base material: silica
Particle size (mean): 4 µm
Pore size (mean): 25 nm
Functional group: Diol
pH stability: 2.5-7.5
Calibration range: 1.0 x 104 - 5× 10Da
(globular proteins)


Protein Calibration Curves

SEC_SuperSW2000-ProteinsCalibration_rev.png              

Columns: TSKgel SuperSW columns,
4.6 mm ID × 30 cm
Mobile phase: 0.2 mol/L phosphate buffer, pH 6.7
Flow rate: 0.35 mL/min
Detection: UV @ 280 nm
Samples: standard proteins
(5 µL, 0.1 g/L each)
1. thyroglobulin
2. γ-globulin
3. bovine serum albumin
4. β-lactoglobulin
5. lysozyme
6. cytochrome C
7. glycine tetramer

PEO and PEG Calibration Curves

SEC_SuperSW2000-PEO-PEGCalibration_rev.png

Columns: TSKgel SuperSW columns,
4.6 mm ID × 30 cm
Mobile phase: 0.05% sodium azide aqueous solution
Flow rate: 0.35 mL/min
Detection: RI
Temperature: 25 °C
Samples: polyethylene oxides (PEO) standards
polyethylene glycols (PEG) standards
(5 µL)

 

                  

Analysis of Standard Proteins at Low Level Concentrations

 

SEC_SuperSW3000-Fig18.png

 

Columns: TSKgel SuperSW3000, 4 µm,
2 mm ID × 30 cm
Competitor 200 PC 3.2/30, 13 µm,
3.2 mm ID × 30 cm
Mobile phase: 0.1 mol/L phosphate buffer + 0.1 mol/L Na2SO4 + 0.05% NaN3, pH 6.7
Detection: UV @ 280 nm
Injection vol.: 0.2 µL
Samples: 1. thyroglobulin (1.0 g/L)
2. β-globulin (2.0 g/L)
3. ovalbumin (2.0 g/L)
4. ribonuclease A (3.0 g/L)
5. p-aminobenzoic acid (0.02 g/L)

 


Analysis of Proteins in Human Serum

SEC_SuperSW3000-Fig21.png

Column: TSKgel SuperSW3000, 4 µm,
1 mm ID × 30 cm
Mobile phase: 50 mmol/L NaH2PO4 + 0.5 mol/L NaCl, pH 7.0
Flow rate: 8 µL/min
Detection: UV @ 280 nm
Temperature: ambient
Sample: serum (x 10), 1 µL

Fraction (1 mL) was directly loaded to SELDI chip H50. The chip was washed and desalted then applied to MS. 

This data is courtesy of Dr. Majima, Protenova

 

For additional applications, please see our applications database.

 

Code #DescriptionLiterature Type
AN004 Mobile Phase Optimization for the Analysis of an Antibody-Based Fusion Protein and Aggregates using a TSKgel Super SW3000 Size-Exclusion ColumnApplication Note
AN013 The Characterization of Biological Samples by Microbore TSKgel SuperSW3000 Size Exclusion ColumnsApplication Note
AN015 Stability of TSKgel SuperSW3000 for Analyzing Proteins by SECApplication Note
AN087 Characterization of Native and PEGylated Fab-arms Application Note
AN102 Characterization of a Novel Antibody Drug Conjugate Mimic by Size Exclusion and Hydrophobic Interaction ChromatographyApplication Note
AO01 Gel Filtration Chromatography with TSKgel columns Application Overview
DS1157 TSKgel SuperSW3000 ProductsOperating Conditions and Specifications
IM01 TSKgel Column Instruction ManualInstruction Manual
IM20 Regeneration of TSKgel® SEC-SW HPLC ColumnsInstruction Manual
LCAT01A TSKgel U/HPLC Columns 2019 Product Guide -- SEC, SW Series ColumnsProduct Catalog
PD12 Guarding Column LifetimePerformance Data
PO06 TSKgel SuperSW2000 and SuperSW3000 columnsProduct Overview
SR095 TSKgel SuperSW SeriesSeparation Report
TP117 High sensitivity protein separations using a new size exclusion chromatography microcolumn for use in conjunction with MSTechnical Presentation
TP164 Detection of protein heterogeneity by HPLCTechnical Presentation
TP190 Characterization of Two Novel Analytical Chromatographic Columns for Orthogonal Analysis of Monoclonal Antibodies and Protein Aggregates and Their IsoformsTechnical Presentation
TP207 Characterization of Native and PEGylated Fab-arms using Various Modes of Analytical ChromatographyTechnical Presentation
P/NDescriptionParticle Size Housing MaterialID (mm)Length (cm)Price ($) 
18762TSKgel SuperSW2000 and SuperSW3000 Guard Column for 4.6 mm ID columns4Stainless Steel4.63.5639