HPLC Columns

TSKgel SuperAW4000

The TSKgel SuperAW4000 column is packed with rigid, porous, polymer beads which show minimal shrinkage or swelling in polar organic solvents (methanol, acetonitrile, DMSO, isopropanol, THF, and HFIP).  This column is stable in a wide variety of organic solvents at concentrations up to 100%.

The main application area for the TSKgel SuperAW4000 column is the analyses of polymers that are soluble in polar organic solvents up to 400,000 Da. Examples include cellulose derivatives, polyimide, and sodium dodecylsulfate (all in 10 mmol/L LiBr in DMF), cleansing gel in methanol, and degree of saponification of polyvinylalcohol in hexafluoroisopropanol (HFIP). Reductions in analysis time and mobile phase consumption due to the small column dimensions (6 mm ID x 15 cm) make the TSKgel SuperAW4000 column ideal for high throughput applications.


P/NDescriptionParticle Size Housing MaterialID (mm)Length (cm)Price ($) 
19317TSKgel SuperAW40006Stainless Steel6151,642

Product Attributes

Matrix: hydrophilic methacrylate
Particle size (mean): 6 µm
Pore size (mean): 45 nm
Exclusion limits
    PEO in H2O: 4 × 105
    PS in DMF with
    10 mmol/L LiBr:
1 × 106
    PEG in MeOH with
    10 mmol/L LiBr:
3 × 10​6




Polyethylene Oxide, Polyethylene Glycol, and Polystyrene Calibration Curves


Columns: TSKgel SuperAW columns,
6.0 mm ID × 15 cm
Mobile phase: A. H2O
B. MeOH with 10 mmol/L LiBr
C. DMF with 10 mmol/L LiBr
Flow rate: 0.6 mL/min
Detection: RI
Temperature: 25°C
Samples: A. polyethylene oxide (PEO),
B. polyethylene glycol (PEG),and
C. ethylene glycol (EG) standards


Column Efficiency of TSKgel SuperAW Columns





Column: TSKgel SuperAW columns,
6.0 mm ID × 15 cm
Mobile phase: H2O
Flow rate: 0.6 mL/min
Detection: RI
Temperature: 25 °C
Injection vol.: 5 µL (2.5 g/L)
Sample: ethylene glycol

Code #DescriptionLiterature Type
AN005 High-Throughput SEC of Water Soluble Polymers Using TSKgel SuperAW ColumnsApplication Note
AO01 Gel Filtration Chromatography with TSKgel columns Application Overview
DS1188 TSKgel SuperAW4000 ProductsOperating Conditions and Specifications
IM01 TSKgel Column Instruction ManualInstruction Manual
LCAT01C TSKgel U/HPLC Columns 2019 Product Guide -- SEC, Alpha and SuperAW ColumnsProduct Catalog
PO07 TSKgel SuperAW ColumnsProduct Overview
SR099 TSKgel SuperAW Series High-performance SEC Semi-Micro ColumnsSeparation Report
P/NDescriptionParticle Size Housing MaterialID (mm)Length (cm)Price ($) 
19321TSKgel SuperAW2500-4000 Guard Column for 6.0 mm ID columns7Stainless Steel4.63.5553