HPLC Columns

TSKgel G4000Hxl

The TSKgel G4000Hxl column is packed with spherical particles composed of polystyrene crosslinked with divinylbenzene (PS-DVB). This column is for the non-aqueous separation of polymers with molecular weights of less than 400,000 Da.  The main characteristics of the TSKgel G4000Hxl column are: ultra-low sample adsorption, i.e., the column shows true size exclusion behavior for most polymers, limited solvent range, and a maximum operating temperature of 80 °C.

As the number of solvents that can be exchanged directly with the shipping solvent (THF) of the TSKgel G4000Hxl column is limited, use TSKgel Hhr columns when a broader range of solvents is needed.



P/NDescriptionParticle Size Housing MaterialID (mm)Length (cm)Price ($) 
16137TSKgel G4000Hxl5Stainless Steel7.8301,766

Product Attributes

Matrix: polystyrene-divinylbenzene
Particle size (mean): 5 µm
Pore size (mean): 20 nm
Exclusion limit: 4.0 × 105​ Da
Max. temperature: 80° C

Polystyrene Calibration Curves


Columns: TSKgel HXL columns,
7.8 mm ID × 30 cm
Mobile phase: THF
Flow rate: 1.0 mL/min
Detection: UV @ 254 nm
Temperature: 25 °C
Samples: polystyrene standards


Separation of Polystyrene Standards



Columns: TSKgel G4000HXL, 5 µm,
7.8 mm ID × 30 cm
Mobile phase: THF
Flow rate: 1.0 mL/min
Detection: UV @ 254 nm
Samples: polystyrene standards
1. 1.126 × 106 Da
2. 1.86 × 105 Da
3. 4.28 × 10Da
4. 1.67 × 104 Da
5. 1.02 × 104 Da
6. 2,800 Da
7. 890 Da
8. benzene, 78 Da

For additional applications, please see our applications database.


Code #DescriptionLiterature Type
DS1214 TSKgel G4000Hxl ProductsOperating Conditions and Specifications
IM01 TSKgel Column Instruction ManualInstruction Manual
LCAT01D TSKgel U/HPLC Columns 2019 Product Guide -- SEC, H Series ColumnsProduct Catalog
SR028 Principles and Applications of High Performance GPC : TSKgel Hxl Type Columns for Oligomer Analysis Separation Report
P/NDescriptionParticle Size Housing MaterialID (mm)Length (cm)Price ($) 
07113TSKgel G1000Hxl-G4000Hxl Guard Column for 7.8 mm ID columns8Stainless Steel64610