HPLC Columns

TSKgel SuperHZ2500

TSKgel SuperHZ2500 columns are packed with rigid, porous, 3 μm polymer beads. These ultra-fine particles provide high resolution for the non-aqueous separation of solvent-soluble small molecules and oligomers less than 20,000 Da. The maximum operating temperature for the TSKgel SuperHZ2500 columns are 60 °C.  The THF shipping solvent may be replaced by a limited number of organic solvents.
TSKgel SuperHZ2500 columns are ideal for high throughput, high efficiency GPC applications such as those encountered in combinatorial chemistry experiments. These columns feature ultra-low sample adsorption, i.e., the columns show true size exclusion behavior for most polymers.
TSKgel SuperHZ2500 column dimensions are 6 mm ID × 15 cm and 4.6 mm ID × 15 cm versus 7.8 mm ID × 30 cm for conventional GPC columns. The smaller column dimensions translate to a reduction of peak volume by a factor of 3.4 (6 mm ID) and a factor of 5.8 (4.6 mm ID) versus the same component eluting from a corresponding TSKgel G2500Hxl column. Thus, your HPLC system may require optimization of components that can give rise to extra-column band broadening, such as connecting tubing, injector, injection volume, detector cell volume, and detector time constant.



P/NDescriptionParticle Size Housing MaterialID (mm)Length (cm)Price ($) 
19311TSKgel SuperHZ25003Stainless Steel4.6151,771
19304TSKgel SuperHZ25003Stainless Steel6151,692

Product Attributes

Matrix: polystyrene-divinylbenzene
Particle size (mean): 3 µm
Pore size (mean): 3 nm
Exclusion limit: 2.0 × 10Da
Max. temperature: 60 °C

Polystyrene Calibration Curves


Columns: TSKgel SuperHZ columns,
4.6 mm ID × 15 cm
Mobile phase: THF
Flow rate: 0.35 mL/min
Detection: UV @ 254 nm
Temperature: 25 °C
Injection vol.: 2 µL
Samples: polystyrene standards


Comparison of Analysis on TSKgel SuperHZ and TSKgel HXL Columns



Columns: A: TSKgel SuperHZ columns
(4000, 3000, 2500),
4.6 mm ID × 15 cm ×3
B:TSKgel HXL columns
(4000, 3000, 2500),
7.8 mm ID ×30 cm ×3
Mobile phase: THF
Flow rate: A. 0.35 mL/min  B. 1.0 mL/min
Detection: RI
Temperature: 40 °C
Injection vol.: A. 5 µL  B. 30 µL
Samples: phenolic resin

Code #DescriptionLiterature Type
AN006 High-Throughput GPC Analysis of Phenolic Resin Using TSKgel SuperHZ ColumnsApplication Note
DS1196 TSKgel SuperHZ2500 ProductsOperating Conditions and Specifications
IM01 TSKgel Column Instruction ManualInstruction Manual
LCAT01D TSKgel U/HPLC Columns 2019 Product Guide -- SEC, H Series ColumnsProduct Catalog
SR101 SuperHZ Series Ultra-High Throughput GPC ColumnsSeparation Report
P/NDescriptionParticle Size Housing MaterialID (mm)Length (cm)Price ($) 
19314TSKgel SuperHZ1000-4000 and HZM-N and -M Guard Column for 4.6 mm ID columns3Stainless Steel4.62570
19666TSKgel SuperHZ1000-4000 and HZM-N and -M Guard Column for 6 mm ID columns3Stainless Steel4.63.5570