Separation of Large DNA Fragments
| Columns: |
TSKgel DNA-STAT, 5 µm,
4.6 mm ID × 10 cm
|
| Mobile phase: |
A: 20 mmol/L Tris-HCl, pH 8.5
B: 0.75 mol/L NaCl in buffer A |
| Gradient: |
0 min (50% B) 25 min (75% B) |
| Flow rate: |
0.8 mL/min |
| Detection: |
UV @ 260 nm |
|
|
Separation of Large DNA Fragments Containing more than 10kb
| Columns: |
TSKgel DNA-STAT, 5 µm,
4.6 mm ID × 10 cm
|
| Mobile phase: |
A: 20 mmol/L Tris-HCl, pH 8.5
B: 1.0 mol/L NaCl, pH 8.5 |
| Gradient: |
B) 75 to 100% (20 min.) |
| Flow rate: |
0.5 mL/min |
| Detection: |
UV @ 260 nm |
| Sample: |
1 kb Ladder |
|
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