Products

HPLC Columns

TSKgel BioAssist S

Specially designed for the separation of large biomolecules such as antibodies, the large pores (130 nm) of the TSKgel BioAssist S cation exchange columns offer superior capacity and resolution at a low column pressure drop. Constructed via a polymerization technique that allows an equivalent density of sulfopropyl ionic exchange groups to be incorporated into the particle without reducing pore size, the TSKgel BioAssist S columns are unlike other ion exchange columns that use graft polymerization for polymer chain introduction. The TSKgel BioAssist S columns’ large pores are very accessible even for high molar mass proteins. This leads to higher chromatographic efficiency and binding capacity for purification.

 

The standard TSKgel BioAssist S column is 4.6 mm ID x 5 cm, while a 10 mm ID x 10 cm semi-preparative column is available for scale-up. Both columns are made of PEEK to reduce protein adsorption.

 

 
P/NDescriptionParticle Size Housing MaterialID (mm)Length (cm)Price ($) 
19686TSKgel BioAssist S7PEEK4.651,363
21411TSKgel BioAssist S13PEEK10105,197
             

Product Attributes

Matrix: polymethacrylate
Particle size (mean): 7 µm and 13 µm
Pore size (mean): 130 nm
Functional group: sulfopropyl
Counter ion: NA+
pH stability: 2.0 - 12.0
Capacity (gamma globulin):

70

Small ion capacity: 0.1 eq/L
pKa: 2.4

 

 

 

 

                 

Analysis of Bromelain

IEX_BioAssist-S-Fig25.png

Columns: A: TSKgel BioAssist S, 7 µm,
4.6 mm ID × 5 cm
B: Competitor S, 5 mm ID × 5 cm
Mobile phase: 20 min (TSKgel) or 30 min (Competitor S) linear gradient of NaCl from 0 to 0.5 mol/L in 20 mmol/L sodium phophate buffer, pH 7.0
Flow rate: 0.8 mL/min for TSKgel; 1.0 mL/min for Competitor S
Detection: UV @ 280 nm
Temperature: 25 °C
Samples: crude bromelein (C4882, Sigma), 1 mg in 100 L

 

Analysis of Protein standards

IEX_BioAssist-S-Fig27.png

Column: A: TSKgel BioAssist S, 7 µm,
4.6 mm ID × 5 cm

B: Conventional S type product C,
5.0 mm ID × 5 cm
C: Conventional S type product D,
4.6 mm ID × 5 cm
Mobile phase: A: 20 mmol/L sodium phosphate buffer, pH 6.5
B: 20 mmol/L sodium phosphate buffer containing 1.0 mol/L NaCl, pH 6.5
Gradient: 32 min (A-B)
Flow rate: 0.8 mL/min
Detection: UV @ 280 nm
Temperature: 10 °C
Injection vol.: 20 µL
Samples: 1. myoglobin 1 g/L
2. α-chymotrypsinogen, 2 g/L
3. ribonuclease A, 4 g/L
4. cytochrome C, 2 g/L
5. lysozyme, 2 g/L

 

 

Separation of IgM by Cation Exchange Chromatography

IEX_BioAssist-S-Fig26rev.png

 

Columns: TSKgel BioAssist S, 7 µm,
4.6 mm ID x 5 cm

Mobile phase: 20 mmol/L sodium phosphate buffer,
pH 6.0
Gradient: 0 mol/L - 0.3 mol/L NaCl (5 min)
0.3 mol/L - 0.5 mol/L NaCl (10 min)
Flow rate: 1 mL/min
Detection: UV @ 280 nm
Sample: 500 µL of 9.5 mg/mL IgM in mouse ascites fluid; shaded peaks represent albumin and IgM respectively

 

Analysis of Peptides

IEX_BioAssist-S-Fig28.png

 

Column: TSKgel BioAssist S, 7 µm,
4.6 mm ID × 5 cm

Mobile phase: A: 20 mmol/L sodium acetate buffer,
pH 5.0
B: 20 mmol/L sodium acetate buffer containing 1 mol/L NaCl, pH 5.0
Gradient: A to B linear gradient (20 min)
Detection: UV @ 280 nm
Temperature: 25 °C

For additional applications, please see our applications database.

 

Code #DescriptionLiterature Type
AN008 Ion Exchange Analysis of Monoclonal Antibodies on Large Pore TSKgel BioAssist ColumnsApplication Note
AN009 Separation of IgM using TSKgel BioAssist SApplication Note
DS1184 TSKgel BioAssist S ProductsOperating Conditions and Specifications
IM01 TSKgel Column Instruction ManualInstruction Manual
PO04 TSKgel BioAssist Ion Exchange ColumnsProduct Overview
SR100 TSKgel BioAssist Series Ion Exchange ColumnsSeparation Report
TP100 Purification of Immunoglobulin M using a novel ion-exchanger (BioAssist Q and S)Technical Presentation
TP102 The Characteristics of Novel Semi-Preparative Ion-Exchange ColumnsTechnical Presentation