Products

HPLC Columns

TSKgel Ether-5PW Glass

TSKgel Ether-5PW Glass is the glass format of the TSKgel Ether-5PW column. This column is based on a 100 nm pore size polymethacrylate base material bonded with polyether groups.  The TSKgel Ether-5PW Glass column is stable in either acid or caustic cleaning regimens and provides excellent access to larger molecules with low diffusion coefficients.

TSKgel Ether-5PW Glass is less hydrophobic than TSKgel Phenyl-5PW and the best choice for the separation of very hydrophobic proteins or monoclonal antibodies.

 

 
P/NDescriptionParticle Size Housing MaterialID (mm)Length (cm)Price ($) 
14013TSKgel Ether-5PW Glass10Glass551,227
14014TSKgel Ether-5PW Glass10Glass87.51,581
          

Product Attributes

Pore size (mean): 100 nm
Particle size (mean): 10 µm
pH stability: 2.0-12.0
Functional group: ether

Structure:

HIC_Ether5PW_structure.png

     

Screening of Mouse Monoclonal Antibodies

 

HIC_Ether-5PW-Fig2C.png



 

Column: TSKgel Ether-5PW, 10 μm,
8.0 mm ID × 7.5 cm, glass

Mobile phase: linear gradient from A to B as shown
A: 0.05 mol/L sodium phosphate,
pH 7.0, 2.0 mol/L ammonium sulfate, 1.0 mol/L glycine
B: 0.05 mol/L sodium phosphate,
pH 7.0, 1.0 mol/L glycine
Flow rate: 10 mL/min
Detection: UV @ 280 nm
Samples: A: 20 μL unequilibrated mouse
IgG2b κ ascites
B: 20 μL unequilibrated mouse
IgM κ ascites

For additional applications, please see our applications database.

 
Code #DescriptionLiterature Type
DS1036 TSKgel Ether-5PW ProductsOperating Conditions and Specifications
IM01 TSKgel Column Instruction ManualInstruction Manual
LCAT03 TSKgel U/HPLC Columns 2019 Product Guide -- HIC ColumnsProduct Catalog
P/NDescriptionParticle Size Housing MaterialID (mm)Length (cm)Price ($) 
14025TSKgel Ether-5PW glass Glass Guardgel Kit for 5 mm ID and 8 mm ID columns20Glass 0771