TSKgel Phenyl-5PW Glass

TSKgel Phenyl-5PW Glass is the glass format of the TSKgel Phenyl-5PW column for samples that may adsorb to stainless steel. This column is based on a 100 nm pore size polymethacrylate base material bonded with phenyl groups. The TSKgel Phenyl-5PW Glass column is stable in either acid or caustic cleaning regimens and provides excellent access to larger molecules with low diffusion coefficients.

Being the most hydrophobic among the three TSKgel HIC columns, TSKgel Phenyl-5PW Glass is an excellent choice to screen for the selectivity, retention and recovery of most biomolecules.

P/N	Description	Particle Size (µm)	Housing Material	ID (mm)	Length (cm)	Price ($)
0013063	TSKgel Phenyl-5PW Glass	10	Glass	5	5	1,489	Inquire
0008804	TSKgel Phenyl-5PW Glass	10	Glass	8	7.5	1,918	Inquire

Product Attributes

Pore size (mean):	100 nm
Particle size:	10 µm
pH stability:	2.0 - 12.0
Functional group:	phenyl

Structure:

Purification of Myristoylated Frequenin

Column:	TSKgel Phenyl-5PW, 10 μm, 5 mm ID × 5 cm, glass
Mobile phase:	equilibration in 50 mmol/L HEPES, 100 mmol/L KCI, 1 mmol/L DTT, 1 mmol/L MgCl₂, 1 mmol/L CaCl₂, pH 7.5 step gradient at 12.5 min to 50 mmol/L HEPES, 1 mmol/L DTT, 1 mmol/L MgCl₂, 2 mmol/L EGTA, pH 7.5
Flow rate:	1 mL/min
Detection:	UV @ 280 nm
Temperature:	ambient
Sample:	E. coli lysate containing myristoylated frequenin, 100 μL

For additional applications, please see our applications database.

Code #	Description	Literature Type
DS1037	TSKgel Phenyl-5PW Products	Operating Conditions and Specifications
IM01	TSKgel Column Instruction Manual	Instruction Manual
LCAT03	TSKgel U/HPLC Columns 2019 Product Guide -- HIC Columns	Product Catalog

HPLC Columns

TSKgel Phenyl-5PW Glass

Product Attributes

Structure:

Purification of Myristoylated Frequenin