TSKgel Phenyl-5PW

TSKgel Phenyl-5PW is based on a 100 nm pore size polymethacrylate base material bonded with phenyl groups. The TSKgel Phenyl-5PW column is stable in either acid or caustic cleaning regimens and provides excellent access to larger molecules with low diffusion coefficients.

Being the most hydrophobic among the three TSKgel HIC columns, TSKgel Phenyl-5PW is an excellent choice to screen for the selectivity, retention and recovery of most biomolecules. Besides the stainless steel standard column format, glass and PEEK column housings are available for samples that may adsorb to stainless steel.

P/N	Description	Particle Size (µm)	Housing Material	ID (mm)	Length (cm)	Price ($)
0018759	TSKgel Phenyl-5PW	10	Stainless Steel	2	7.5	1,446	Inquire
0007573	TSKgel Phenyl-5PW	10	Stainless Steel	7.5	7.5	1,918	Inquire
0007656	TSKgel Phenyl-5PW	13	Stainless Steel	21.5	15	6,501	Inquire

Product Attributes

Pore size (mean):	100 nm
Particle size:	10 µm and 13 µm
pH stability:	2.0 - 12.0
Functional group:	phenyl

Structure:

Proteins: Scale up to Preparative Separations

Column:	A: TSKgel Phenyl-5PW, 10 μm, 7.5 mm ID × 7.5 cm B: TSKgel Phenyl-5PW, 13 μm, 21.5 mm ID × 15 cm
Mobile phase:	60 min linear gradient from 1.8 mol/L to 0 mol/L (NH₄)₂SO₄ in 0.1 mol/L phosphate buffer, pH 7.0
Flow rate:	0.5 mL/min (7.5 mm ID) or 4 mL/min (21.5 mm ID)
Detection:	UV @ 280 nm
Temperature:	25 °C
Sample:	1. myoglobin 2. ribonuclease 3. lysozyme 4. α-chymotrypsinogen 5. α-chymotrypsin

For additional applications, please see our applications database.

Code #	Description	Literature Type
DS1037	TSKgel Phenyl-5PW Products	Operating Conditions and Specifications
IM01	TSKgel Column Instruction Manual	Instruction Manual
LCAT03	TSKgel U/HPLC Columns 2019 Product Guide -- HIC Columns	Product Catalog
SR074	Separation of Monoclonal Antibodies Using TSKgel HPLC Columns	Separation Report
SR097	Application of TSKgel Semi-Micro for High-Sensitivity Analyses	Separation Report

P/N	Description	Particle Size (µm)	Housing Material	ID (mm)	Length (cm)	Price ($)
0019308	TSKgel Guard Cartridge Holder for 2 mm ID cartridges	0		2	1	506
0042155	TSKgel Phenyl-5PW Guard Cartridge for 2 mm ID column	10	Stainless Steel	2	1	0
0007652	TSKgel Phenyl-5PW Guardgel Kit for 7.5 mm ID column	20	Stainless Steel		0	878
0016095	TSKgel Phenyl-5PW Guardgel Kit for 21.5 mm ID column	20	Stainless Steel		0	1350

HPLC Columns

TSKgel Phenyl-5PW

Product Attributes

Structure:

Proteins: Scale up to Preparative Separations