Proteins: Scale up to Preparative Separations
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|
| Column: |
A: TSKgel Phenyl-5PW, 10 μm,
7.5 mm ID × 7.5 cm
B: TSKgel Phenyl-5PW, 13 μm,
21.5 mm ID × 15 cm
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| Mobile phase: |
60 min linear gradient from 1.8 mol/L to 0 mol/L (NH4)2SO4 in 0.1 mol/L phosphate buffer, pH 7.0
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| Flow rate: |
0.5 mL/min (7.5 mm ID) or
4 mL/min (21.5 mm ID)
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| Detection: |
UV @ 280 nm |
| Temperature: |
25 °C |
| Sample: |
1. myoglobin
2. ribonuclease
3. lysozyme
4. α-chymotrypsinogen
5. α-chymotrypsin |
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For additional applications, please see our applications database.