Products

HPLC Columns

TSKgel BioAssist Chelate

TSKgel BioAssist Chelate is the PEEK format of the TSKgel Chelate-5PW column. This column  is based on a 100 nm (1000 Å) pore size polymethacrylate base material covalently bonded with iminodiacetic acid (IDA) groups.  Prior to chromatography, a metal ion, such as Zn2+, Ni2+ or Cu2+, is added to form a chelate with the IDA group. Peptides and proteins containing histidine residues will normally adsorb to these chelated ions at neutral pH. The retained compounds can be eluted with buffer containing imidazole or glycine.

 

 
P/NDescriptionParticle Size Housing MaterialID (mm)Length (cm)Price ($) 
20022TSKgel BioAssist Chelate10PEEK7.851,486
        

Product Attributes

Pore size (mean): 100 nm
Exclusion limit
(base resin, estimate):
<1.0 × 10Da globular proteins
Ligand concentration: 20 µmol/L resin
Particle size: 10 µm 
pH stability: 2.0 - 12.0
Functional group: iminodiacetic acid

Structure:

AFC_Chelate_structure.png


Code #DescriptionLiterature Type
DS1048 TSKgel Chelate-5PW ProductsOperating Conditions and Specifications
DS1207 TSKgel BioAssist ChelateOperating Conditions and Specifications
IM01 TSKgel Column Instruction ManualInstruction Manual