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HPLC Columns

TSKgel Boronate-5PW Glass

TSKgel Boronate-5PW Glass is the glass format of the TSKgel Boronate-5PW column. This column is based on a 100 nm pore size polymethacrylate base material bonded with m-aminophenyl boronate.  This ligand is capable of forming a tetrahedral boronate anion under alkaline pH conditions. This anionic structure can bind with 1,2 cis-diol groups such as those found in carbohydrates, glycosylated proteins, and catecholamines. Interaction between the boronate anion and the 1,2 cis-diol groups is enhanced in the presence of Mg2+ ions and is inhibited by amine-containing buffers.  Adsorption onto the TSKgel Boronate-5PW matrix takes place in basic buffers, such as HEPES and morpholine, while desorption takes place in carbohydrate or amine-containing mobile phase like sorbitol or Tris. Applications include nucleic acids, catecholamines, nucleosides, and nucleotides.

 

 
P/NDescriptionParticle Size Housing MaterialID (mm)Length (cm)Price ($) 
14449TSKgel Boronate-5PW Glass10Glass551,375
        

Product Attributes

Pore size (mean): 100 nm
Exclusion limit
(base resin, estimate):
<1.0 × 10Da globular proteins
Adsorption capacity: 40 µmol/L resin
Particle size: 10 µm 
pH stability: 2.0 - 9.0
Functional group: m-aminophenyl boronate

Structure:

AFC_Boronate_structure.png


Code #DescriptionLiterature Type
DS1073 TSKgel Boronate-5PW ProductsOperating Conditions and Specifications
IM01 TSKgel Column Instruction ManualInstruction Manual
LCAT06 TSKgel U/HPLC Columns 2019 Product Guide -- AFC ColumnsProduct Catalog
P/NDescriptionParticle Size Housing MaterialID (mm)Length (cm)Price ($) 
14451TSKgel Boronate-5PW Glass Guardgel Kit for 5 mm ID column20Glass 0743