TSKgel Chelate-5PW Glass

TSKgel Chelate-5PW Glass is the glass format of the TSKgel Chelate-5PW column. This column is based on a 100 nm pore size polymethacrylate base material covalently bonded with iminodiacetic acid (IDA) groups. Prior to chromatography, a metal ion, such as Zn²⁺, Ni²⁺ or Cu²⁺, is added to form a chelate with the IDA group. Peptides and proteins containing histidine residues will normally adsorb to these chelated ions at neutral pH. The retained compounds can be eluted with buffer containing imidazole or glycine.

P/N	Description	Particle Size (µm)	Housing Material	ID (mm)	Length (cm)	Price ($)
0014440	TSKgel Chelate-5PW Glass	10	Glass	5	5	1,549	Inquire

Product Attributes

Pore size (mean):	100 nm
Exclusion limit (base resin, estimate):	<1.0 × 10⁷Da globular proteins
Ligand concentration:	20 µmol/L resin
Particle size:	10 µm
pH stability:	2.0 - 12.0
Functional group:	iminodiacetic acid

Structure:

Analysis of Yeast Enzymes

Column:	TSKgel Chelate-5PW, 10 µm, 8 mm ID × 7.5 cm, glass
Metal ions:	Zn²⁺, saturated
Mobile phase:	40 min linear pH gradient from 20 mmol/L HEPES-MES-acetic acid, pH 8.0, to 20 mmol/L HEPES-MES-acetic acid, pH 4.0, both in 0.5 mol/L NaCl
Flow rate:	1.0 mL/min
Detection:	UV @ 280 nm
Recovery:	G6PDH: 90%, and ADH: 97% of enzymatic activity
Sample:	yeast enzyme concentrate
Purification:	G6PDH: 8.7 fold, and ADH: 3.9 fold

*G6PDH: glucose-6-phosphate dehydrogenase
*ADH: alcohol dehydrogenase

For additional applications, please see our applications database.

Code #	Description	Literature Type
DS1048	TSKgel Chelate-5PW Products	Operating Conditions and Specifications
IM01	TSKgel Column Instruction Manual	Instruction Manual
LCAT06	TSKgel U/HPLC Columns 2019 Product Guide -- AFC Columns	Product Catalog

HPLC Columns

TSKgel Chelate-5PW Glass

Product Attributes

Structure:

Analysis of Yeast Enzymes