Products

HPLC Columns

TSKgel Chelate-5PW Glass

TSKgel Chelate-5PW Glass is the glass format of the TSKgel Chelate-5PW column. This column is based on a 100 nm pore size polymethacrylate base material covalently bonded with iminodiacetic acid (IDA) groups.  Prior to chromatography, a metal ion, such as Zn2+, Ni2+ or Cu2+, is added to form a chelate with the IDA group. Peptides and proteins containing histidine residues will normally adsorb to these chelated ions at neutral pH. The retained compounds can be eluted with buffer containing imidazole or glycine.

 

 
P/NDescriptionParticle Size Housing MaterialID (mm)Length (cm)Price ($) 
14440TSKgel Chelate-5PW Glass10Glass551,238
        

Product Attributes

Pore size (mean): 100 nm
Exclusion limit
(base resin, estimate):
<1.0 × 10Da globular proteins
Ligand concentration: 20 µmol/L resin
Particle size: 10 µm 
pH stability: 2.0 - 12.0
Functional group: iminodiacetic acid

Structure:

AFC_Chelate_structure.png


   

Analysis of Yeast Enzymes

 

AFC_Chelate-5PW-Fig3C.png


 



Column: TSKgel Chelate-5PW, 10 µm,
8 mm ID × 7.5 cm, glass
Metal ions: Zn2+, saturated
Mobile phase: 40 min linear pH gradient from 20 mmol/L HEPES-MES-acetic acid, pH 8.0, to 20 mmol/L HEPES-MES-acetic acid, pH 4.0, both in 0.5 mol/L NaCl
Flow rate: 1.0 mL/min
Detection: UV @ 280 nm
Recovery: G6PDH*: 90%, and ADH*: 97% of enzymatic activity
Sample: yeast enzyme concentrate
Purification: G6PDH: 8.7 fold, and ADH: 3.9 fold

*G6PDH: glucose-6-phosphate dehydrogenase
*ADH: alcohol dehydrogenase

For additional applications, please see our applications database.

 
 
Code #DescriptionLiterature Type
DS1048 TSKgel Chelate-5PW ProductsOperating Conditions and Specifications
IM01 TSKgel Column Instruction ManualInstruction Manual
LCAT06 TSKgel U/HPLC Columns 2019 Product Guide -- AFC ColumnsProduct Catalog