Products

HPLC Columns

TSKgel Boronate-5PW

TSKgel Boronate-5PW is based on a 100 nm pore size polymethacrylate base material bonded with m-aminophenyl boronate.  This ligand is capable of forming a tetrahedral boronate anion under alkaline pH conditions. This anionic structure can bind with 1,2 cis-diol groups such as those found in carbohydrates, glycosylated proteins, and catecholamines. Interaction between the boronate anion and the 1,2 cis-diol groups is enhanced in the presence of Mg2+ ions and is inhibited by amine-containing buffers.  Adsorption onto the TSKgel Boronate-5PW matrix takes place in basic buffers, such as HEPES and morpholine, while desorption takes place in carbohydrate or amine-containing mobile phase like sorbitol or Tris.  Applications include nucleic acids, catecholamines, nucleosides, and nucleotides.

 

 
P/NDescriptionParticle Size Housing MaterialID (mm)Length (cm)Price ($) 
13066TSKgel Boronate-5PW10Stainless Steel7.57.51,470
        

Product Attributes

Pore size (mean): 100 nm
Exclusion limit
(base resin, estimate):
<1.0 × 10Da globular proteins
Adsorption capacity: 40 µmol/L resin
Particle size: 10 µm 
pH stability: 2.0 - 9.0
Functional group: m-aminophenyl boronate

Structure:

AFC_Boronate_structure.png


   

Isocratic Separation of Nucleosides


AFC_Boronate-5PW-Fig1B.png

 

 

Column: TSKgel Boronate-5PW, 10 µm,
7.5 mmID × 7.5 cm
Mobile phase: 0.1 mol/L phosphate buffer, pH 8.0
Flow rate: 1.0 mL/min
Detection: UV @ 280 nm
Samples: 1. cytidine
2. uridine
3. guanosine
4. adenosine



For additional applications, please see our applications database.

 
Code #DescriptionLiterature Type
DS1073 TSKgel Boronate-5PW ProductsOperating Conditions and Specifications
IM01 TSKgel Column Instruction ManualInstruction Manual
LCAT06 TSKgel U/HPLC Columns 2019 Product Guide -- AFC ColumnsProduct Catalog
TP157 Analyses of glycated human serum albumin by high performance boronate affinity chromatography coupled with online post column reaction detectionTechnical Presentation
P/NDescriptionParticle Size Housing MaterialID (mm)Length (cm)Price ($) 
13125TSKgel Boronate-5PW Guardgel Kit for 7.5 mm ID column20Stainless Steel 0695