Products

HPLC Columns

TSKgel Amide-80, 10 µm

TSKgel Amide-80, 10 µm columns are packed with spherical silica particles that are covalently bonded with carbamoyl groups. The amide stationary phase provides a unique selectivity under regular normal phase conditions or in the hydrophilic interaction mode of chromatography.

TSKgel Amide-80, 10 µm columns possess superior stability in aqueous/organic solvent systems, an advantage over other hydrophilic stationary phases such as traditional amino-bonded phase columns. In addition, TSKgel Amide-80, 10 µm columns are ideally suited for sensitive LC/MS analysis of water-soluble polar compounds because the high organic content in the mobile phase provides rapid evaporation of solvent during electrospray ionization.

Target applications for the TSKgel Amide-80, 10 µm columns include the analysis of saccharides, glycans, oligosaccharides, peptides, and polar compounds from natural products.  

The pore size of the bonded phase is indicated by the number in the product description, in this case TSKgel Amide-80 has 8 nm nominal pore size. The nominal pore size of the starting base silica is 10 nm.

 

 
P/NDescriptionParticle Size Housing MaterialID (mm)Length (cm)Price ($) 
14460TSKgel Amide-80, 10 µm10Stainless Steel21.5304,423
14459TSKgel Amide-80, 10 µm10Stainless Steel7.8301,742
     

Product Attributes

Pore size (silica): 8 nm*
Particle size (mean): 10 µm
pH stability: 2.0-7.5
Functional group: carbamoyl
Max. temperature (°C): 80
Surface area(m2/g) 450

*The pore size of the bonded phase is indicated by the number in the product description, in this case TSKgel Amide-80 has 8 nm nominal pore size. The nominal pore size of the starting base silica is 10 nm.

Structure:

NPC_Amide80_structure.jpg

 
Code #DescriptionLiterature Type
DS1052 TSKgel Amide-80 ProductsOperating Conditions and Specifications
IM01 TSKgel Column Instruction ManualInstruction Manual
LCAT04 TSKgel U/HPLC Columns 2019 Product Guide -- HILIC ColumnsProduct Catalog
PD06 TSKgel Amide-80 Columns for the Analysis of Hydrophilic CompoundsPerformance Data
SR055 Separation of Saccharides Using TSKgel Amide-80, a Packing Material for High-Performance Normal Phase Partition Chromatography (part 1)Separation Report
SR079 Separation of Saccharides Using TSKgel Amide-80, a Packing Material for High Performance Normal Phase Partition Chromatography (part 2)Separation Report
P/NDescriptionParticle Size Housing MaterialID (mm)Length (cm)Price ($) 
14461TSKgel Amide-80 Guard Column for 21.5 mm ID, 10 µm column10Stainless Steel21.57.51268