HPLC Columns

TSKgel Octadecyl-2PW

TSKgel Octadecyl-2PW is based on a highly cross-linked polymethacrylate base matrix bonded with octadecyl groups. The polymeric matrix provides excellent stability in high pH buffer systems and can withstand rigorous cleaning with either acid or base. The 12.5 nm (125 Å) pore size of TSKgel Octadecyl-2PW columns makes them ideally suited for peptides and small proteins. They demonstrate faster analysis than other competitive reversed phase polymeric columns.


P/NDescriptionParticle Size Housing MaterialID (mm)Length (cm)Price ($) 
18754TSKgel Octadecyl-2PW5Stainless Steel215784
17500TSKgel Octadecyl-2PW5Stainless Steel4.615814
17501TSKgel Octadecyl-2PW5Stainless Steel6151,131

Product Attributes

Pore size (mean): 12.5 nm
Exclusion limit: 8,000 Da
Particle size (mean): 5 µm
pH stability: 2.0-12.0
Functional group: C18 (monomeric bonding chemistry)




Separation of Eight Peptides



Columns: TSKgel Octadecyl-2PW, 5 µm,
4.6 mm ID × 15 cm

Mobile phase: 30 min linear gradient from 0.1% TFA/CH3CN from 90/10 to 30/70
Flow rate: 1.0 mL/min
Detection: UV @ 215 nm
Temperature: ambient
Samples: 1. met-enkephalin
2. bradykinin
3. leu-enkaphalin
4. neurotensin
5. bombesin
6. angiotensin I
7. somatostatin
8. insulin (bovine)

For additional applications, please see our applications database.


Code #DescriptionLiterature Type
DS1106 TSKgel Octadecyl-2PW ProductsOperating Conditions and Specifications
IM01 TSKgel Column Instruction ManualInstruction Manual
LCAT05B TSKgel U/HPLC Columns 2019 Product Guide -- RPC Columns, Polymer-BasedProduct Catalog
SR097 Application of TSKgel Semi-Micro for High-Sensitivity Analyses Separation Report
P/NDescriptionParticle Size Housing MaterialID (mm)Length (cm)Price ($) 
19308TSKgel Guard Cartridge Holder for 2 mm ID cartridges0 21403
42161TSKgel Octadecyl-2PW Guard Cartridge for 2 mm ID column, 3 pk5Stainless Steel21448
17502TSKgel Octadecyl-2PW Guard column for 4.6 mm ID column5Stainless Steel4.61468
17503TSKgel Octadecyl-2PW Guard column for 6 mm ID column5Stainless Steel61488