HPLC Columns

TSKgel Octadecyl-4PW

TSKgel Octadecyl-4PW is based on a large pore, highly cross-linked polymethacrylate base matrix bonded with octadecyl groups. The polymeric matrix provides excellent stability in high pH buffer systems and can withstand rigorous cleaning with either acid or base.  The large pore size of TSKgel Octadecyl-4PW columns, 50 nm (500Å), allows unhindered access to proteins and other large molar mass biopolymers. The particle size offerings allow for analytical and semi-preparative scale separations.


P/NDescriptionParticle Size Housing MaterialID (mm)Length (cm)Price ($) 
18755TSKgel Octadecyl-4PW7Stainless Steel215765
13351TSKgel Octadecyl-4PW7Stainless Steel4.615727
16257TSKgel Octadecyl-4PW13Stainless Steel21.5153,844

Product Attributes

Pore size (mean): 50 nm
Exclusion limit: 1,000 - 2.0 × 105 Da
Estimated ligand density: 1 eq/L
Particle size (mean): 7.0µm  and 13 µm
pH stability: 2.0-12.0
Functional group: C18 (monomeric bonding chemistry)





Comparison of pH Conditions


Column: TSKgel Octadecyl-4PW, 5 µm,
4.6 mm ID × 15 cm

Mobile phase: A: 0.2% TFA, pH 1.9
B: 0.05 mol/L phosphate buffer, pH 7.1
C: 0.02 mol/L NH3, pH 10.8
Gradient:  50 min. linear gradient from 0% to 80% CH3CN 
Flow rate: 1.0 mL/min
Detection: UV @ 220 nm
Samples: 1. met-enkephalin
2. bradykinin
3. leu-enkephalin
4. neurotensin
5. bombesin
6. angiotensin I
7. somatostatin
8. insulin


For additional applications, please see our applications database.


Code #DescriptionLiterature Type
DS1039 TSKgel Octadecyl-4PW ProductsOperating Conditions and Specifications
IM01 TSKgel Column Instruction ManualInstruction Manual
LCAT05B TSKgel U/HPLC Columns 2019 Product Guide -- RPC Columns, Polymer-BasedProduct Catalog
SR097 Application of TSKgel Semi-Micro for High-Sensitivity Analyses Separation Report
P/NDescriptionParticle Size Housing MaterialID (mm)Length (cm)Price ($) 
19308TSKgel Guard Cartridge Holder for 2 mm ID cartridges0 21403
19018TSKgel Guard Cartridge Holder for 3.2 mm ID cartridges0 3.21.5404
42160TSKgel Octadecyl-4PW Guard Cartridge for 2 mm ID column, 3 pk7Stainless Steel21448
19008TSKgel Octadecyl-4PW Guard Cartridge for 4.6 mm ID column, 3 pk7Stainless Steel3.21.5302