HPLC Columns

TSKgel Octadecyl-NPR

TSKgel Octadecyl-NPR is based on non-porous polymethacrylate particles of uniform 2.5 μm size, which provides high efficiency separations and fast analyses of peptides and proteins. The highly cross-linked polymethacrylate base material provides excellent stability in high pH buffer systems and can withstand rigorous cleaning with either acid or base.

NPR stands for Non-Porous Resin.


P/NDescriptionParticle Size Housing MaterialID (mm)Length (cm)Price ($) 
14005TSKgel Octadecyl-NPR2.5Stainless Steel4.63.51,074


Product Attributes

Pore size (mean): non-porous
Exclusion limit: >1.0 × 106 Da
Estimated ligand density: 1 eq/L
Particle size (mean): 2.5 µm
pH stability: 2.0-12.0
Functional group: C18 (monomeric bonding chemistry)





Analysis and Recovery of Nanogram Protein Samples


Column: TSKgel Octadecyl-NPR, 2.5 µm, 4.6 mm ID × 3.5 cm
Mobile phase: 10 min linear gradient from 15% to 80% CH3CN in 5 mmol/L HCIO4
Flow rate: 1.5 mL/min
Detection: UV @ 220 nm
Samples: 50 ng each of:
1. ribonuclease A
2. insulin
3. cytochrome C
4. lysozyme
5. transferrin
6. myoglobin
Note: Blank gradient trace also shown

For additional applications, please see our applications database.

Code #DescriptionLiterature Type
DS1041 TSKgel Octadecyl-NPR ProductsOperating Conditions and Specifications
IM01 TSKgel Column Instruction ManualInstruction Manual
LCAT05B TSKgel U/HPLC Columns 2019 Product Guide -- RPC Columns, Polymer-BasedProduct Catalog