Separation of Antioxidants and UV Absorbants
| Column: |
TSKgel ODS-100V, 5 µm,
4.6 mm ID × 25 cm
|
| Mobile phase: |
A: H2O
B: CH3CN |
| Gradient: |
0 min (60%B) 20 min (100%B) |
| Flow rate: |
1.0 mL/min |
| Temperature: |
50 °C
|
| Detection: |
UV @ 225 nm |
| Injection vol.: |
10 µL
|
| Concentration: |
10 mg/L each
|
| Samples: |
1. Cyasorb® UV-24 2. BHA
3. Ionox 100 4. Seesorb 101
5. Tinuvin® P 6. Yoshinox SR
7. Seesorb 202 8. BHT
9. Noclizer M-17
10. Yoshinox 2246R 11. Topanol®
12. Yoshinox 425 13. Cyanox®
14. Cyasorb UV-531 15. Ionox 220
16. Nonflex CBP 17. Tinuvin 326
18. Tinuvin 120 19. Iranox®
20. Uvtex OB 21. Tinuvin 327
22.Tinuvin 328 23. Irganox 1010
24. Irganox 1330 25. Irganox 1076
26. Irgafos® 168 |
Separation of Organic Acids
| Column: |
TSKgel ODS-100V, 5 µm, 4.6 mm ID × 25 cm
|
| Mobile phase: |
0.1% H3PO4 |
| Flow rate: |
1.0 mL/min |
| Temperature: |
40 °C |
| Injection vol.: |
10 µL
|
| Samples: |
1. oxalic acid (0.1 g/L)
2. I-Tartartic acid (0.5 g/L)
3. formic acid (1.0 g/L)
4. I-Malic acid (1.0 g/L)
5. I-Ascorbic Acid (0.1 g/L)
6. lactic acid (1.0 g/L)
7. acetic acid (1.0 g/L)
8. maleic acid (0.1 g/L)
9. citric acid (1.0 g/L)
10. succinic acid (1.0 g/L)
11. fumaric acid (0.025g/L)
12. acrylic acid (0.1 g/L)
13. propionic acid (2.0 g/L)
14. glutaric acid (1.0 g/L)
15. itaconic acid (0.025 g/L) |
|
|
Separation of Water and Lipid-soluble Vitamins
| Column: |
TSKgel ODS-100V, 5 µm,
4.6 mm ID × 15 cm
|
| Mobile phase: |
A: 0.1% TFA in H2O
B: 0.1% TFA in ACN |
| Gradient: |
0 min (0%B) 20 min (40%B)
22 min (100%B) 50 min (100%B) |
| Flow rate: |
1.0 mL/min |
| Detection: |
UV @ 280 nm |
| Temperature: |
40 °C |
| Injection vol.: |
5 µL
|
| Samples: |
1. L-ascorbic acid
2. nicotinic acid
3. thiamine
4. pyridoxal
5. pyridoxine
6. caffeine
7. riboflavine
8. retinol
9. δ-tocopherol
10. α- tocopherol
11. α- tocopherol acetate |
|
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