Products

HPLC Columns

TSKgel UP-SW2000

TSKgel UP-SW2000 columns are designed for the analysis of small proteins, peptides and oligonucleotides. The columns are packed with 2 μm silica-based beads shielded with a hydrophilic diol-type bonded phase that prevents the silica surface from interacting with protein samples.


TSKgel UP-SW2000 columns feature the same pore size as the well-established TSKgel SuperSW2000 columns. Hence methods developed using TSKgel SuperSW2000 columns can easily be transferred to TSKgel UP-SW2000 columns on conventional HPLC systems as well as on UHPLC systems.


TSKgel UP-SW2000 columns offer higher resolution, improved peak shape and increased efficiency yielding methods that are robust, reproducible and easily transferrable between UHPLC and HPLC systems.


TSKgel UP-SW2000 columns offer superior reproducibility injection-to-injection, from column-to-column within the same lot and from lot-to-lot. %RSD values for peak parameters including retention times, area peak height, peak asymmetry, and theoretical plate count demonstrate the exceptional reproducibility of these columns.

 

 
P/NDescriptionParticle Size Housing MaterialID (mm)Length (cm)Price ($) 
23515TSKgel UP-SW20002Stainless Steel4.6151,709
23514TSKgel UP-SW20002Stainless Steel4.6302,303

          

Product Attributes

Base material: silica
Particle size (mean): 2 µm
Pore size (mean): 12.5 nm
Functional group: Diol
pH stability: 2.5 - 7.5
Calibration range (proteins): 1,000 - 1.5 ×105 Da
(globular proteins)


Protein Calibration Curves

TSKgel UP-SW calibration curves

Columns: A. TSKgel UP-SW2000, 2 µm,
     4.6 mm ID × 30 cm
B. TSKgel UP-SW3000, 2 µm,
     4.6 mm ID × 30 cm
C. TSKgel UP-SW Aggregate, 3 µm,
     4.6 mm ID × 30 cm
Mobile phase: 100 mmol/L phosphate buffer, pH 6.7, + 100 mmol/L Na2SO4 + 0.05% NaN3
Flow rate: 0.35 mL/min
Detection: UV @ 280 nm
Temperature: 25 °C
Injection vol.: 10 µL
Samples: 1. thyroglobulin (MW 640,000 Da)
    (1' thyroglobulin dimer)
2. γ-globulin (MW 155,000 Da)
    (2' γ-globulin dimer)
3. ovalbumin (MW 47,000 Da)
4. riboneclease A (MW 13,000 Da)
5. p-aminobenzoic acid (MW 137 Da)

                    

Analysis of Oligonucleotides

 

Analysis of Oligonucleotides

 

Column: TSKgel UP-SW2000, 2 µm,
4.6 mm ID × 30 cm × 2
Mobile phase: 0.05% NaN3 and 0.3 mol/L NaCl in 0.05 mol/L phosphate, pH 6.7
Flow rate: 0.2 mL/min
Detection: UV @ 260 nm
Temperature: 25 °C
Sample: 20 mer: 5’-GAATTCATCGGTTCAGAGAC-3’
19 mer: 5’-AATTCATCGGTTCAGAGAC-3’

 

Analysis of Small Protein and Peptide Mixture

 

Analysis of Small Protein and Peptide Mixture

 

Column: TSKgel UP-SW2000, 2 µm,
4.6 mm ID × 30 cm
Mobile phase: 100 mmol/L phosphate buffer, pH 6.7,
100 mmol/L Na2SO4, 0.05% NaN3
Flow rate: 0.35 mL/min
Instruments: Agilent 1100 with Chemstation v. 2.18.18
Detection: UV @ 280 nm
Temperature: 25 °C
Injection vol.: 5 µL
Samples: Peptide 1: Myoglobin 16.7 kDa
Peptide 2: RPDFC LEPPY TGPCK ARIIR YFYNA KAGLC QTFVY GGCRA KRNNF KSAED CMRTC GGA (58 aa) 6.5 kDa
Peptide 3: QRLGNQWAVGHLM (14 aa)
1.6 kDa

 


For additional applications, please see our applications database.

 

Code #DescriptionLiterature Type
AN115 Analysis of Oligonucleotides by SECApplication Note
AN119 Characterization of Recombinant Protein Biotherapeutic using TSKgel UP-SW2000 Size Exclusion Chromatography Column In-Line with LenS3 MALS DetectorApplication Note
DS1244 TSKgel UP-SW2000 ProductsOperating Conditions and Specifications
IM20 Regeneration of TSKgel® SEC-SW HPLC ColumnsInstruction Manual
PD21 Increased Sensitivity with LenS3 MALS DetectorPerformance Data
TP227 Evaluation of a New 2 μm Silica-Based Size Exclusion Chromatography Column for the Analysis of Proteins, mAb Fragments and PeptidesTechnical Presentation
P/NDescriptionParticle Size Housing MaterialID (mm)Length (cm)Price ($) 
23516TSKgel UP-SW2000 Guard Column for 4.6 mm ID column2Stainless Steel4.62670
23517TSKgel UP-SW2000 DC Guard Column for 4.6 mm ID column2Stainless Steel4.62670