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TOYOPEARL AF-Amino-650M

TOYOPEARL AF-Amino-650M is a reactive resin that requires activation for ligand attachment.  The resin may be used to couple ligands using their carboxyl groups through peptide bond formation or aldehyde groups by reductive amination. Aldehyde groups may be present in a carbohydrate or glycoprotein ligand or may be introduced into the ligand by mild, periodate oxidation.

The optimized functional group density of TOYOPEARL AF-Amino-650M (100 μmol/mL) is ideal for coupling of either proteins or low molecular weight ligands. For example, lactose was coupled by reductive alkylation to yield a ligand density of ca. 30 μmol/mL resin.

 

 
P/NDescriptionResin VolumeParticle Size (µm)   Price
($)
 
43411TOYOPEARL AF-Amino-650M10 mL65  128
08002TOYOPEARL AF-Amino-650M25 mL65  260
08039TOYOPEARL AF-Amino-650M100 mL65  741
18074TOYOPEARL AF-Amino-650M1 L65  -
18316TOYOPEARL AF-Amino-650M5 L65  -
      

Product Attributes


Pore size (mean): 100 nm
Particle size (mean): 65 µm
Pressure rating: 0.3 MPa
Shipping buffer: 20% ethanol
pH stability: 2-13
Shelf life: 10 years

 

Structure:

AFC_AF-Amino-650M_Structure.png

 

 

       

Coupling procedure


AFC_AF-Amino_coupling.png

 

Representative coupling densities

Protein coupled g/L resin
soybean trypsin inhibitor 5.8
ovalbumin 6.7
bovine serum albumin 19.2
human IgG 6.7
cytochrome 3.3
lysozyme 5.8

coupling agent: NaCNBH3 or carbodiimide
optimal pH: 4.5 - 6.0

Code #DescriptionLiterature Type
IM02 Instruction Manual for TOYOPEARL and TSKgel PW-type ResinsInstruction Manual
IM05 Instruction Manual -- TOYOPEARL AF-Amino-650MInstruction Manual
PCAT04 TOYOPEARL, TSKgel and Ca⁺⁺Pure-HA Bulk Resin 2019 Product Guide -- AffinityProduct Catalog
PO13 TOYOPEARL AF-Amino-650M ResinProduct Overview
RIS0001 TOYOPEARL AF-Amino-650MResin Information Sheet
P/NDescriptionPrice ($) 
43410TOYOPEARL AFFIPAK326