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TOYOPEARL MX-Trp-650M

TOYOPEARL MX-Trp-650M is a high capacity, multimodal cation exchange resin used for the purification of monoclonal antibodies and other proteins. The tryptophan ligand has both weak carboxyl cation exchange and indole hydrophobic functional groups. The selectivity of the resin can be adjusted through control of binding or elution pH, ionic strength, salt type and additives.

TOYOPEARL MX-Trp-650M maintains dynamic binding capacity (DBC) at elevated feedstock or buffer conductivities. It maintains a high DBC even after extended CIP (clean in place) cycles using 0.5 mol/L NaOH. Applications include the separation of antibody aggregates and monomer in mAb purification polishing steps.

 

 
P/NDescriptionResin VolumeParticle Size (µm)   Price
($)
 
22817TOYOPEARL MX-Trp-650M25 mL75  210
22818TOYOPEARL MX-Trp-650M100 mL75  660
22819TOYOPEARL MX-Trp-650M1 L75  -
22820TOYOPEARL MX-Trp-650M5 L75  -
      

Product Attributes

Pore size (mean): 100 nm
Particle size (mean): 75 µm
Pressure rating: 0.3 MPa
Shipping buffer: 20% ethanol
pH stability: 3-13


Structure:

MM_MX-Trp-650M_Structure.png

 
     

Purification of Monoclonal Antibodies

Separation of mAb monomers and aggregates

MX-Trp-650M-Fig14_rev.png

Resin: TOYOPEARL MX-Trp-650M
Column size: 6.6 mm ID × 2 cm
Mobile phase: Buffer A: 0.1 mol/L acetate + 0.2 mol/L NaCl, pH 4.3
Buffer B: 0.1 mol/L acetate + 0.4 mol/L NaCl, pH 5.6
Flow rate: 150 cm/hr (0.86 mL/min)
Detection: UV @ 280 nm, conductivity (mS/cm)
Sample: 10 mg mAb + mAb aggregates
Sample load: 1 g/L

 

SEC chromatograms of the collected fractions

MX-Trp-650M-Fig15_rev.png

Resin: TOYOPEARL MX-Trp-650M
Column size: 6.6 mm ID × 2 cm
Mobile phase: Buffer A: 0.1 mol/L acetate + 0.2 mol/L NaCl, pH 4.3
Buffer B: 0.1 mol/L acetate + 0.4 mol/L NaCl, pH 5.6
Flow rate: 150 cm/hr (0.86 mL/min)
Detection: UV @ 280 nm, conductivity (mS/cm)
Sample: 10 mg mAb + mAb aggregates
Sample load: 1 g/L
      

Stability

Stability in 0.5 mol/L NaOH

MX-Trp-650M-Fig2.png

Resin: TOYOPEARL MX-Trp-650M
Alkaline cleaning (CIP) conditions
3CV: 0.5 mol/L NaOH
5CV: 0.1 mol/L Tris-HCl, pH 8.5 + 0.3 mol/L NaCl
Flow rate: 212 cm/hr (1.0 mL/min)

DBC Measurement
Column size: 6 mm ID × 4 cm
Binding buffer: 0.05 mol/L acetate buffer, pH 4.7 + 0.1 mol/L NaCl
Flow rate: 212 cm/hr (1.0 mL/min)
Detection: UV @ 280 nm
Sample: polyclonal human IgG
Sample load: 1 mg/mL

 

Dynamic Binding Capacity

Effect of buffer pH and salt on DBC

MX-Trp-650M-Fig3.png

Resin: TOYOPEARL MX-Trp-650M
Column size: 6 mm ID × 4 cm
Mobile phase: Buffer A: 0.05 mol/L acetate buffer, pH 4.0 - 6.0 + 0 - 0.2 mol/L NaCl
Buffer B: 0.1 mol/L Tris-HCl buffer, pH 8.5 + 0.3 mol/L NaCl
Flow rate: 212 cm/hr (1.0 mL/min)
Detection: UV @ 280 nm
Sample: polyclonal human IgG
Sample load: 1 mg/mL

Dynamic binding capacity (DBC) calculated from 10% height of breakthrough curve

Elution Kinetics

Narrow elution peak widths

MX-Trp-650M-Fig5.png

Resin: TOYOPEARL MX-Trp-650M
Column size: 6 mm ID × 4 cm 
Mobile phase: Buffer A: 0.05 mol/L acetate buffer, pH 4.7 + 0.1 mol/L NaCl (12 mS/cm)
Buffer B: 0.1 mol/L Tris-HCl buffer, pH 8.5 + 0.3 mol/L NaCl
Flow rate: A: 212 cm/hr (1.0 mL/min)
B: 424 cm/hr (2.0 mL/min) started at 124 min
Detection: UV @ 280 nm
Sample: CHO cell culture media, monoclonal antibody
Sample load: 1 mg/mL diluted with buffer A

 

 

Code #DescriptionLiterature Type
AN044 TOYOPEARL MX-Trp-650M Resin SelectivityApplication Note
AN045 TOYOPEARL MX-Trp-650M Salt Selectivity and ToleranceApplication Note
IM02 Instruction Manual for TOYOPEARL and TSKgel PW-type ResinsInstruction Manual
PCAT05 TOYOPEARL, TSKgel and Ca⁺⁺Pure-HA Bulk Resin 2019 Product Guide -- Mixed-ModeProduct Catalog
PD11 Development of a High Capacity, Mixed-Mode Resin for High Conductivity mAb FeedstocksPerformance Data
PO32 TOYOPEARL MX-Trp-650MProduct Overview
RIS0038 TOYOPEARL MX-Trp-650MResin Information Sheet
TP178 A New Mixed-Mode Resin for Large Scale Biomolecule Purification Technical Presentation
P/NDescriptionPrice ($) 
OC41MDTRP-96Resin Seeker TOYOPEARL MMC 96-well plate kit286
45231SkillPak Mixed-Mode 1 mL column library319
45265SkillPak Mixed-Mode 5 mL column library444
45224SkillPak TOYOPEARL MX-Trp-650M 1 mL columns (qty. 5)253
45261SkillPak TOYOPEARL MX-Trp-650M 5 mL column210
22824ToyoScreen MX-Trp-650M, 1 mL (qty. 6)402
22825ToyoScreen MX-Trp-650M, 5 mL (qty. 6)936
45051ToyoScreen RoboColumns MX-Trp-650M, 200 µL260
45052ToyoScreen RoboColumns MX-Trp-650M, 600 µL340