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Ca⁺⁺Pure-HA

Ca⁺⁺Pure-HA is a hydroxyapatite resin used in the chromatographic separation of biomolecules. Unlike other resins available from Tosoh Bioscience, Ca⁺⁺Pure-HA is both the ligand and the base bead.

Key applications for use of Ca⁺⁺Pure-HA include the purification of monoclonal and polyclonal antibodies, including IgG, IgA and IgM, IgG Fab regions, the purification of antibody fragments, the isolation of single-stranded from double-stranded DNA and the purification of fusion and phosphoproteins. 

Ca⁺⁺Pure-HA resin has been sintered at high temperatures for increased mechanical and pH stability, allowing it to withstand the rigors of industrial-scale applications. Ca⁺⁺Pure-HA has a demonstrated dynamic binding capacity (DBC), at 5% breakthrough, of 55 mg mAb/mg resin at 5 minute residence time. 

Ca⁺⁺Pure-HA provides exceptional separation properties and unequalled selectivity and resolution for multiple classes of biomolecules. The highly selective and robust nature of Ca⁺⁺Pure-HA offers the flexibility to use this resin at any stage in a process from capture to final polishing.

 

 
P/NDescriptionResin VolumeParticle Size (µm)   Price
($)
 
45045Ca⁺⁺Pure-HA50 g39  333
45039Ca⁺⁺Pure-HA100 g39  659
45040Ca⁺⁺Pure-HA250 g39  -
45041Ca⁺⁺Pure-HA500 g39  -
45042Ca⁺⁺Pure-HA1 kg39  -
45043Ca⁺⁺Pure-HA5 kg39  -
           

Product Attributes

Particle size (mean): 39 µm
Pressure rating: 10 MPa
Shipped as: dry powder
pH stability: 6.5 - 14
Shelf life: 10 years


Structure:

HA_CaPure-HA_Structure.png

      

Stability

Mechanical stability

HA_CaPure-Fig4_rev2.png

Resin: Ca⁺⁺Pure-HA
Column size: 1.1 cm ID × 19.3 cm (dry pack)
2.2 cm ID × 20.3 cm (dry pack)
4.4 cm ID × 19.3 cm (dry pack)
2.2 cm ID × 20.6 cm (wet pack)
10.0 cm ID × 21.3 cm (wet pack)
Mobile phase: 5 mmol/L sodium phosphate, 
pH 7.2
Linear velocity: as noted
Detection: pressure (MPa)

 

Caustic stability

HA_CaPure-Fig3_rev.png

Dynamic Binding Capacity

Lysozyme

HA_CaPure-Fig6_rev.png

Resin: Ca⁺⁺Pure-HA
Column size: 6.6 cm ID × 3 cm (1 mL)
Mobile phase: 5 mmol/L sodium phosphate,
pH 6.8
Flow rate: 5 min residence time: 0.21 mL/min
(36 cm/hr)
3.5 min residence time: 0.3 mL/min
(53 cm/hr)
2 min residence time: 0.52 mL/min
(91 cm/hr)
1 min residence time: 1.0 mL/min
(180 cm/hr)
Detection: UV @ 280 nm (10% breakthrough)
Sample: lysozyme, 1.0 g/L

 

Human IgG

HA_CaPure-Fig7_rev.png

Resin: Ca⁺⁺Pure-HA
Column size: 6.6 cm ID × 3 cm (1 mL)
Mobile phase: 10 mmol/L sodium phosphate,
pH 6.5
Flow rate: 5 min residence time: 0.21 mL/min
(36 cm/hr)
2 min residence time: 0.52 mL/min
(91 cm/hr)
Detection: UV @ 280 nm (10% breakthrough)
Sample: human IgG, 1.0 g/L


1 min 2 min 3.5 min 5 min
Lysozyme 24.9 g/L 24.9 g/L
25.0 g/L
25.1 g/L
Human IgG 32.4 g/L
N/A N/A 51.6 g/L

Code #DescriptionLiterature Type
AN107 Effective removal of mAb aggregate using Ca⁺⁺Pure-HA media with potassium saltsApplication Note
IM16 Instruction Manual -- Ca⁺⁺Pure-HA Resin Packing and Use GuideInstruction Manual
PCAT06 TOYOPEARL, TSKgel and Ca⁺⁺Pure-HA Bulk Resin 2018 Product Guide -- HydroxyapatiteProduct Catalog
PO41 Ca++Pure-HA, Hydroxyapatite Media from Tosoh BioscienceProduct Overview
RDS5040 Ca⁺⁺Pure-HA Resin Operating Conditions and Specifications
P/NDescriptionPrice ($) 
45152MiniChrom Ca⁺⁺Pure-HA233
OC41MDCPHA-500Resin Seeker Ca⁺⁺Pure-HA 96-well plate kits261
OC41MDCPHAResin Seeker Ca⁺⁺Pure-HA 96-well plate kits261
CUS022318CT2ToyoScreen RoboColumns Ca⁺⁺Pure-HA, 200 µL301
CUS1004ToyoScreen RoboColumns Ca⁺⁺Pure-HA, 600 µL326