Products

Process Media

TOYOPEARL HW-40F

TOYOPEARL HW-40F resin is a hydroxylated methacrylic polymer for size exclusion chromatography. TOYOPEARL HW-40F resin has a 5 nm pore size and a 45 µm bead size. Surface hydroxyl groups render this resin very hydrophilic, therefore minimal non-specific adsorption occurs, making the TOYOPEARL HW-40F resin useful for protein separations. It is typically used for high throughput desalting and buffer exchange. It also can remove surfactants such as Triton X100 from protein solutions. It is often used for smaller biomolecules with exclusion limits up to 10,000 Da.

 

The good mechanical stability of the TOYOPEARL HW-40F resin produces excellent flow characteristics in large industrial size columns (up to 0.3 MPa). TOYOPEARL HW-40F resin is chemically stable from pH

2-14, and it is this stability that allows a constant packing volume over a wide range of salt concentrations and cleaning in place (CIP) with acid or base. Also, this resin can be run at elevated temperatures (4 - 60 °C) and is autoclavable at 121 °C.

 

If higher resolution is needed, the smaller particle TOYOPEARL HW-40S resin is preferred for process SEC.

 

 
P/NDescriptionResin VolumeParticle Size (µm)   Price
($)
 
19808TOYOPEARL HW-40F150 mL45  222
07448TOYOPEARL HW-40F500 mL45  515
14682TOYOPEARL HW-40F1 L45  -
07968TOYOPEARL HW-40F5 L45  -
          

Product Attributes

Pore size (mean): 5 nm
Exclusion limit: 10,000 Da
Particle size (mean): 45 µm
Pressure rating: 0.3 MPa
Shipping buffer: 20% ethanol
pH stability: 2-13
Shelf life: 10 years

 

Molar Mass Separation Ranges

Polyethylene glycols
and oxides
100 - 3,000 Da
Dextrans 100 - 7,000 Da
Globular proteins 100 - 1 × 10Da

Structure:

SEC_HWresin_structure.png

 
 

Calibration Curves for Globular Proteins

SEC_HW-40_HW-50_HW55-Fig2_rev.png

Resins: A. TOYOPEARL HW-40
B. TOYOPEARL HW-50
C. TOYOPEARL HW-55
D. TOYOPEARL HW-65
E. TOYOPEARL HW-75
Column size: 22 mm ID × 30 cm
Mobile phase: 0.06 mol/L phosphate buffer, pH 7.0, + 0.06 mol/L KCl
Detection: UV @ 280 nm
Temperature: ambient
Samples: protein standards
Legend: Ve= elution volume
Vc= column volume
 
    

Desalting of Bovine Serum Albumin


SEC_HW-40F-Fig7.png

Resin: TOYOPEARL HW-40F
Column size: 7.5 mm ID × 30 cm
Mobile phase: A. distilled water
B. 10 mmol/L ammonium formate
C. 50 mmol/L ammonium formate
Flow rate: 48 cm/hr (0.4 mL/min)
Detection: UV @ 220 nm
Temperature: 25 °C
Sample: 2.5 mL of:
1. bovine serum albumin (0.1%)
2. sodium acetate (10%)


For additional applications, please see our applications database.

 
 
Code #DescriptionLiterature Type
IM02 Instruction Manual for TOYOPEARL and TSKgel PW-type ResinsInstruction Manual
PCAT01 TOYOPEARL, TSKgel and Ca⁺⁺Pure-HA Bulk Resin 2019 Product Guide -- Size Exclusion Product Catalog
RIS0040 TOYOPEARL HW-40Resin Information Sheet
P/NDescriptionPrice ($) 
45171MiniChrom TOYOPEARL HW-40F-
19821TOYOPEARL SECPAK LMW433