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TOYOPEARL HW-50S

TOYOPEARL HW-50S resin is a hydroxylated methacrylic polymer for size exclusion chromatography. TOYOPEARL HW-50S resin has a 12.5 nm pore size and a 30 µm bead size. Surface hydroxyl groups render this resin very hydrophilic, therefore minimal non-specific adsorption occurs, making the TOYOPEARL HW-50S resin useful for protein separations of biomolecules between 500 and 80,000 Da in size.

 

The good mechanical stability of the TOYOPEARL HW-50S resin produces excellent flow characteristics in large industrial size columns (up to 0.3 MPa). TOYOPEARL HW-50S resin is chemically stable from pH 2-14, and it is this stability that allows a constant packing volume over a wide range of salt concentrations and cleaning in place (CIP) with acid or base. Also, this resin can be run at elevated temperatures (4 - 60 °C) and is autoclavable at 121 °C.

 

If lower resolution is needed, the larger particle TOYOPEARL HW-50F resin can also be used for process SEC.

 

 
P/NDescriptionResin VolumeParticle Size (µm)   Price
($)
 
19811TOYOPEARL HW-50S150 mL30  516
07455TOYOPEARL HW-50S500 mL30  799
07452TOYOPEARL HW-50S1 L30  1,188
14684TOYOPEARL HW-50S5 L30  -
08059TOYOPEARL HW-50S50 L30  -
            

Product Attributes

Pore size (mean): 12.5 nm
Exclusion limit: 80,000 Da
Particle size (mean): 30 µm
Pressure rating: 0.3 MPa
Shipping buffer: 20% ethanol
pH stability: 2-13
Shelf life: 10 years

 

Molar Mass Separation Ranges

Polyethylene glycols
and oxides
100 - 1.8 × 10Da
Dextrans 500 - 2 × 10Da
Globular proteins 500 - 8 × 10Da

Structure:

SEC_HWresin_structure.png

 
 

Calibration Curves for Globular Proteins

SEC_HW-40_HW-50_HW55-Fig2_rev.png

Resins: A. TOYOPEARL HW-40
B. TOYOPEARL HW-50
C. TOYOPEARL HW-55
D. TOYOPEARL HW-65
E. TOYOPEARL HW-75
Column size: 22 mm ID × 30 cm
Mobile phase: 0.06 mol/L phosphate buffer, pH 7.0, + 0.06 mol/L KCl
Detection: UV @ 280 nm
Temperature: ambient
Samples: protein standards
Legend: Ve= elution volume
Vc= column volume
 
 
    

Isolation of a Complex Protein Conjugate


SEC_HW-50S-Fig10.png

Resin: TOYOPEARL HW-50S
Column size: 22 mm ID × 83 cm
Mobile phase: 10 mmol/L HCl
Flow rate: 1.6 cm/hr (01 mL/min)
Detection: UV @ 230 nm
Sample: fraction of crude Tetrahymena H2A containing the ubiquitin-histone conjugate uH2A


For additional applications, please see our applications database.

 
 
Code #DescriptionLiterature Type
IM02 Instruction Manual for TOYOPEARL and TSKgel PW-type ResinsInstruction Manual
PCAT01 TOYOPEARL, TSKgel and Ca⁺⁺Pure-HA Bulk Resin 2019 Product Guide -- Size Exclusion Product Catalog
RIS0040 TOYOPEARL HW-40Resin Information Sheet
RIS0041 TOYOPEARL HW-50Resin Information Sheet
P/NDescriptionPrice ($) 
19820TOYOPEARL SECPAK HP 1,214