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TOYOPEARL HW-55F

TOYOPEARL HW-55F resin is a hydroxylated methacrylic polymer for size exclusion chromatography. TOYOPEARL HW-55F resin has a 50 nm pore size and a 45 µm bead size. Surface hydroxyl groups render this resin very hydrophilic, therefore minimal non-specific adsorption occurs, making the TOYOPEARL HW-55F resin useful for protein separations of biomolecules between 1,000 and 700,000 Da in size.

 

The good mechanical stability of the TOYOPEARL HW-55F resin produces excellent flow characteristics in large industrial size columns (up to 0.3 MPa). TOYOPEARL HW-55F resin is chemically stable from pH 2-14, and it is this stability that allows a constant packing volume over a wide range of salt concentrations and cleaning in place (CIP) with acid or base. Also, this resin can be run at elevated temperatures (4 - 60 °C) and is autoclavable at 121 °C.

 

If higher resolution is needed, the smaller particle TOYOPEARL HW-55S resin can also be used for process SEC.

 

 
P/NDescriptionResin VolumeParticle Size (µm)   Price
($)
 
19812TOYOPEARL HW-55F150 mL45  222
07457TOYOPEARL HW-55F500 mL45  515
14687TOYOPEARL HW-55F1 L45  -
08063TOYOPEARL HW-55F5 L45  -
21918TOYOPEARL HW-55F50 L45  -
            

Product Attributes

Pore size (mean): 50 nm
Exclusion limit: 700,000 Da
Particle size (mean): 45 µm
Pressure rating: 0.3 MPa
Shipping buffer: 20% ethanol
pH stability: 2-13
Shelf life: 10 years

 

Molar Mass Separation Ranges

Polyethylene glycols
and oxides
100 - 1.5 × 10Da
Dextrans 1,000 - 2 × 10Da
Globular proteins 1,000 - 7 × 10Da

Structure:

SEC_HWresin_structure.png

 
 

Calibration Curves for Globular Proteins

SEC_HW-40_HW-50_HW55-Fig2_rev.png

Resins: A. TOYOPEARL HW-40
B. TOYOPEARL HW-50
C. TOYOPEARL HW-55
D. TOYOPEARL HW-65
E. TOYOPEARL HW-75
Column size: 22 mm ID × 30 cm
Mobile phase: 0.06 mol/L phosphate buffer, pH 7.0, + 0.06 mol/L KCl
Detection: UV @ 280 nm
Temperature: ambient
Samples: protein standards
Legend: Ve= elution volume
Vc= column volume
 
 
 

Antibody Separation

Elution profiles of colostral whey

SEC_HW-55F-Fig9_rev.png

Resin: TOYOPEARL HW-55F
Column size: 17 mm ID × 64 cm 
Mobile phase: 25 mmol/L Tris-HCl with 0.3 mol/L NaCl, pH 7.5
Flow rate: 18 cm/hr (0.7 mL/min)
Detection: A. UV @ 280 nm 
B. UV @ 254 nm
Temperature: 23 °C
Samples: 1. unknown
2. IgG1
3. serum albumin
4. β-lactoglobulin (dimer)
5. α-lactalbumin
6-12. unknown


Recovery of Activity

Purification of crude β-galactosidase

SEC_HW-55F-Fig8_rev.png

Resin: TOYOPEARL HW-55F
Column size: 25 mm ID × 55 cm × 2
Mobile phase: 0.2 mol/L phosphate, pH 6.7
Flow rate: 12 cm/hr (1.0 mL/min)
Detection: A. UV @ 280 nm 
B. enzymatic activity
Temperature: 22 °C
Sample: 3 mL containing 45 mg of crude
β-galactosidase

Fraction Yield (%) Specific activity (units/mg) Degree of purification
Original sample
0.95

1 94
2.8 2.9 ×
2 93 3.7
3.9 ×
3 83
6.4 6.7 ×

For additional applications, please see our applications database.

 
    

Comparison of the Elution Volumes of Proteins in Presence and Absence of NaCl


SEC_HW-40-50-55-65-75F-Fig4_rev.png

Resin: TOYOPEARL HW-55F
Column size: 22 mm ID × 50 cm
Mobile phase: 25 mmol/L Tris-HCl with (•) or without () 0.5 mol/L NaCl, pH 7.5
Flow rate: 16 cm/hr (1.0 mL/min)
Detection: UV @ 280 nm, 420 nm for heme proteins, 200 nm for proteins without aromatic amino acids
Temperature: 5-10 °C
Code #DescriptionLiterature Type
IM02 Instruction Manual for TOYOPEARL and TSKgel PW-type ResinsInstruction Manual
PCAT01 TOYOPEARL, TSKgel and Ca⁺⁺Pure-HA Bulk Resin 2019 Product Guide -- Size Exclusion Product Catalog
RIS0042 TOYOPEARL HW-55Resin Information Sheet
P/NDescriptionPrice ($) 
19819TOYOPEARL SECPAK HMW433
19821TOYOPEARL SECPAK LMW433