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TOYOPEARL HW-65S

TOYOPEARL HW-65S resin is a hydroxylated methacrylic polymer for size exclusion chromatography. TOYOPEARL HW-65S resin has a 100 nm pore size and a 30 µm bead size. Surface hydroxyl groups render this resin very hydrophilic, therefore minimal non-specific adsorption occurs, making the TOYOPEARL HW-65S resin useful for protein separations of biomolecules between 40,000 and 5,000,000 Da in size.

 

The good mechanical stability of the TOYOPEARL HW-65S resin produces excellent flow characteristics in large industrial size columns (up to 0.3 MPa). TOYOPEARL HW-65S resin is chemically stable from pH 2-14, and it is this stability that allows a constant packing volume over a wide range of salt concentrations and cleaning in place (CIP) with acid or base. Also, this resin can be run at elevated temperatures (4 - 60 °C) and is autoclavable at 121 °C.

 

If lower resolution is needed, the larger particle TOYOPEARL HW-65F resin can also be used for process SEC.

 

 
P/NDescriptionResin VolumeParticle Size (µm)   Price
($)
 
19815TOYOPEARL HW-65S150 mL30  516
07467TOYOPEARL HW-65S250 mL30  799
07464TOYOPEARL HW-65S500 mL30  1,188
14688TOYOPEARL HW-65S1 L30  -
08068TOYOPEARL HW-65S5 L30  -
18377TOYOPEARL HW-65S50 L30  -
              

Product Attributes

Pore size (mean): 100 nm
Exclusion limit: 5,000,000 Da
Particle size (mean): 30 µm
Pressure rating: 0.3 MPa
Shipping buffer: 20% ethanol
pH stability: 2-13
Shelf life: 10 years

 

Molar Mass Separation Ranges

Polyethylene glycols
and oxides
500 - 1 × 10Da
Dextrans 1 × 104 - 1 × 10Da
Globular proteins 4 × 104 - 5 × 10Da

Structure:

SEC_HWresin_structure.png

 
 

Calibration Curves for Globular Proteins

SEC_HW-40_HW-50_HW55-Fig2_rev.png

Resins: A. TOYOPEARL HW-40
B. TOYOPEARL HW-50
C. TOYOPEARL HW-55
D. TOYOPEARL HW-65
E. TOYOPEARL HW-75
Column size: 22 mm ID × 30 cm
Mobile phase: 0.06 mol/L phosphate buffer, pH 7.0, + 0.06 mol/L KCl
Detection: UV @ 280 nm
Temperature: ambient
Samples: protein standards
Legend: Ve= elution volume
Vc= column volume
 
 
    

Polishing Step for Enzyme Purification


Elution profile of protein and activity

SEC_HW-65S-Fig6_rev.png

Resin: TOYOPEARL HW-65S
Column size: 16 mm ID × 80 cm
Mobile phase: 20 mol/L acetate buffer with 40% ethylene glycol, pH 4.8
Detection: UV @ 280 nm
Temperature: ambient
Sample: concentrated active fractions from TOYOPEARL Phenyl-650 column


For additional applications, please see our applications database.

 
Code #DescriptionLiterature Type
IM02 Instruction Manual for TOYOPEARL and TSKgel PW-type ResinsInstruction Manual
PCAT01 TOYOPEARL, TSKgel and Ca⁺⁺Pure-HA Bulk Resin 2019 Product Guide -- Size Exclusion Product Catalog
RIS0043 TOYOPEARL HW-65Resin Information Sheet
P/NDescriptionPrice ($) 
19820TOYOPEARL SECPAK HP 1,214