News & Events
Contact
Tosoh.com
My Account
Shopping Cart
( 0 )
English
English
option
Choose a search type
Site Search
Product Search
Login
Logout
 
Cart
LOGOUT
Click to collapse
Home
Solutions
GPC Systems
Dual Flow Technology
EcoSEC Elite GPC System
EcoSEC HT GPC System
HPLC Columns
Size Exclusion
Aqueous Size Exclusion - GFC
Organic Size Exclusion - GPC
Ion Exchange
Anion Exchange
Cation Exchange
Reversed Phase
HIC
TSKgel HIC
HIC-ADC
Normal Phase/HILIC
Affinity
Protein A
Fc Receptor Affinity
Process Media
Best-in-Class Resins
By Mode
Affinity
Protein A
HIC
Ion Exchange
Mixed-mode
Size Exclusion
Hydroxyapatite
By Process Step
Capture
Intermediate Purification
Polishing
Process Development
LabPak Media
Resin Seeker 96-Well Plates
SkillPak Columns
ToyoScreen RoboColumns
Multi-Column Chromatography
Virus Particle Analysis
Light Scattering for BioApplications
Products
GPC Systems
Accessories & Standards
Detectors
HPLC Columns
Aqueous Size Exclusion – GFC
SW
SWxl
SuperSW
SW mAb
UP-SW
Desalting Columns
PW
PWxl
PWxl-CP for Cationic Polymers
SuperMultiporePW
Organic Size Exclusion – GPC
Alpha
SuperAW
Hhr
Hxl
SuperH
SuperHZ
SuperMultiporeHZ
High Temperature - HT
Ultra High Temperature - HT2
Cation Exchange
Anion Exchange
Hydrophobic Interaction
Affinity
Reversed Phase
Normal Phase-HILIC
Protein A
Fc Receptor Affinity
Multi-Column Chromatography
Process Media
Cation Exchange
Anion Exchange
Hydrophobic Interaction
Mixed-Mode
Affinity
Size Exclusion
Hydroxyapatite
Protein A
Protein L
Process Development
LabPak
Resin Seeker 96-well Plate Kits
SkillPak Columns
SkillPak 1 BIO
SkillPak 5 BIO
SkillPak 10 BIO
SkillPak 25 BIO
SkillPak 100 BIO
SkillPak 1
SkillPak 5
SkillPak 50
SkillPak 200
ToyoScreen RoboColumns
Accessories and Standards
HPLC Columns Accessories
GPC Systems Accessories
Process Development Accessories
Standards
Product Search
Literature
Advanced Literature Search
Application Note
Application Overview
Brochure
Instruction Manual
Instrument Specifications
Operating Conditions and Specifications
Performance Data
Price List
Product Catalog
Product Overview
Resin Information Sheets
Seminars
Separation Report
Technical Information Sheets
Technical Presentation
White Papers
Applications Database
Service & Support
Customer Service
SDS
Ordering Info
Quotation Request
Custom Column Request
Terms & Conditions
Conditions for Quote
Conditions of Sale
Part Number Change Notification
Technical Support
Resource Center
Chromatography Calculations
Cleaning Methods
Method Optimization
Principles of Chromagraphy
Frequently Asked Questions
RMA Policy
Regulatory Support
Distributors
About Us
What We Do
Locations
Careers
Contact Us
Opt in for Promos
Legal
Terms of Use
Privacy Statement
Conditions of Sale
Conditions of Purchase
Patents
Trademarks
Technical Data
Cookies
ISO Certification
Business Continuity Plan
Quality Policy
Videos
GPC Systems Videos
HPLC Columns Videos
Process Media Videos
Products
Process Media
Home
Tosoh
TOYOPEARL DEAE-650S
GPC Systems
Accessories & Standards
Detectors
HPLC Columns
Aqueous Size Exclusion – GFC
SW
SWxl
SuperSW
SW mAb
UP-SW
Desalting Columns
PW
PWxl
PWxl-CP for Cationic Polymers
SuperMultiporePW
Organic Size Exclusion – GPC
Alpha
SuperAW
Hhr
Hxl
SuperH
SuperHZ
SuperMultiporeHZ
High Temperature - HT
Ultra High Temperature - HT2
Cation Exchange
Anion Exchange
Hydrophobic Interaction
Affinity
Reversed Phase
Normal Phase-HILIC
Protein A
Fc Receptor Affinity
Multi-Column Chromatography
Process Media
Cation Exchange
Anion Exchange
Hydrophobic Interaction
Mixed-Mode
Affinity
Size Exclusion
Hydroxyapatite
Protein A
Protein L
Process Development
LabPak
Resin Seeker 96-well Plate Kits
SkillPak Columns
SkillPak 1 BIO
SkillPak 5 BIO
SkillPak 10 BIO
SkillPak 25 BIO
SkillPak 100 BIO
SkillPak 1
SkillPak 5
SkillPak 50
SkillPak 200
ToyoScreen RoboColumns
Accessories and Standards
HPLC Columns Accessories
GPC Systems Accessories
Process Development Accessories
Standards
Product Search
TOYOPEARL DEAE-650S
TOYOPEARL DEAE-650S
resin is a weak anion exchanger for biomolecule purification. The resin is composed of a base material of hydroxylated methacrylic polymer beads that have been functionalized with diethylaminoethyl (DEAE) weak anion exchange groups. TOYOPEARL DEAE-650S has a high degree of chemical and mechanical stability, allowing cleaning-in-place (CIP) and high flow rates.
TOYOPEARL DEAE-650S has a 100 nm pore size and a 35 µm bead size and can be used for high resolution intermediate purification and polishing process steps. If greater resolution is needed, the smaller particle TSKgel DEAE-5PW resins may be used. If lower resolution is desired, the larger particle size TOYOPEARL DEAE-650M or TOYOPEARL DEAE-650C resins can be used. As both the TOYOPEARL DEAE-650 and TSKgel DEAE-5PW resins use the same ligand and backbone chemistry, the selectivity between the two resins will be unchanged
Quantity:
Order Product
Attributes
Applications
Features
Literature
Accessories
P/N
Description
Resin Volume
Particle Size (µm)
Price($)
0019804
TOYOPEARL DEAE-650S
25 mL
35
217
Inquire
0007472
TOYOPEARL DEAE-650S
250 mL
35
1,562
Inquire
0014692
TOYOPEARL DEAE-650S
1 L
35
-
Inquire
0007973
TOYOPEARL DEAE-650S
5 L
35
-
Inquire
0021483
TOYOPEARL DEAE-650S
50 L
35
-
Inquire
Product Attributes
Base bead:
HW-65
Pore size (mean):
100 nm
Particle size (mean):
35 µm
Ligand type:
weak anion
Ligand pKa:
11.5
DBC:
25 - 35 g/L
Shipping buffer:
20% ethanol
pH stability:
2-13
Structure:
Blood Proteins
Resins:
1. TOYOPEARL DEAE-650S
2. TOYOPEARL DEAE-650M
Column size:
16 mm ID × 15 cm
Mobile phase:
Buffer A: 50 mmol/L Tris-HCl, pH 8.6
Buffer B: 50 mmol/L Tris-HCl, pH 8.6 + 0.5 mol/L NaCl
Gradient:
linear gradient from buffer A to buffer B in 200 mL total volume
Flow rate:
45 cm/hr (1.5 mL/min)
Detection:
UV @ 280 nm
Temperature:
25 °C
Sample:
human serum
Column:
TSKgel G3000SW, 7.5 mm ID
×
30 cm × 2 in series
Mobile phase:
0.1 mol/L phosphate, pH 6.8 + 0.1 mol/L sodium sulfate
Detection:
UV @ 280 nm
Temperature:
25 °C
Samples:
1. crude human serum
2. albumin fraction from TOYOPEARL DEAE-650S
3. albumin fraction from TOYOPEARL DEAE-650M
Kato, Y.; Nakamura, K.; Hashimoto, T. Characterization of TSK-GEL DEAE-Toyopearl 650 Ion Exchanger. J. Chromatogr. 1982, 245, 193-211.
Tryptic Digests
Resin:
TOYOPEARL DEAE-650S
Column size:
6 mm ID × 12 cm
Mobile phase:
pyridine/N-ethyl morpholine
Flow rate:
212 cm/hr (1 mL/min)
Detection:
UV @ 280 nm
Temperature:
ambient
Sample:
enzymatic digest of immunoglobulin L chain
For additional applications, please see our
applications database
.
Scale Up or Down using the Same Ligand
Resins:
TOYOPEARL DEAE-650C, 100µm
TOYOPEARL DEAE-650M, 65 µm
TOYOPEARL DEAE-650S, 35 µm
TSKgel
DEAE-5PW (30), 30µm
TSKgel
DEAE-5PW (20), 20µm
TSKgel DEAE
-5PW, 10µm
Column size:
7.5 mm ID × 7.5 cm
Mobile phase:
Buffer A: 0.02 mol/L Tris-HCl, pH 8.5
Buffer B: 0.5 mol/L NaCl in buffer A
Gradient:
60 min linear gradient from buffer A to buffer B
Flow rate:
136 cm/hr (1.0 mL/min)
Detection:
UV @ 280 nm
Sample:
mAb in mouse ascites (dilution, ×5)
Load volume:
100 µL
Code #
Description
Literature Type
IM02
Instruction Manual for TOYOPEARL and TSKgel PW-type Resins
Instruction Manual
PCAT02
TOYOPEARL, TSKgel and Ca⁺⁺Pure-HA Bulk Resin 2019 Product Guide -- Ion Exchange
Product Catalog
RIS0021
TOYOPEARL DEAE-650
Resin Information Sheet
P/N
Description
Price ($)
0045113
MiniChrom TOYOPEARL DEAE-650S
-
Inquire
0019817
TOYOPEARL IEXPAK HP
486
Inquire