Process Media


TOYOPEARL DEAE-650C resin is a weak anion exchanger for biomolecule purification. The resin is composed of a base material of hydroxylated methacrylic polymer beads that have been functionalized with diethylaminoethyl (DEAE) weak anion exchange groups.  TOYOPEARL DEAE-650C has a high degree of chemical and mechanical stability, allowing cleaning-in-place (CIP) and very high flow rates.

TOYOPEARL DEAE-650C has a 100 nm pore size and a 100 µm bead size and can be used for high throughput capture, intermediate purification, and polishing process steps.

If greater resolution is needed, the smaller particle TOYOPEARL DEAE-650M, TOYOPEARL DEAE-650S, or TSKgel DEAE-5PW resins may be used. As both the TOYOPEARL DEAE-650 and TSKgel DEAE-5PW resins use the same ligand and backbone chemistry, the selectivity between the two resin types will be unchanged. 


P/NDescriptionResin VolumeParticle Size (µm)   Price
07988TOYOPEARL DEAE-650C250 mL100  392
14694TOYOPEARL DEAE-650C1 L100  -
07989TOYOPEARL DEAE-650C5 L100  -
22853TOYOPEARL DEAE-650C50 L100  -

Product Attributes

Base bead: HW-65
Pore size (mean): 100 nm
Particle size (mean): 100 µm
Ligand type: weak anion
Ligand pKa: 11.5
DBC: 25 - 35 g/L
Pressure rating: 0.3 MPa
Shipping buffer: 20% ethanol
pH stability: 2-13





Scale Up or Down using the Same Ligand


Resins: TOYOPEARL DEAE-650C, 100µm
TSKgel DEAE-5PW (30), 30µm
TSKgel DEAE-5PW (20), 20µm
TSKgel DEAE-5PW, 10µm
Column size: 7.5 mm ID × 7.5 cm
Mobile phase: Buffer A: 0.02 mol/L Tris-HCl, pH 8.5
Buffer B: 0.5 mol/L NaCl in buffer A
Gradient: 60 min linear gradient from buffer A to buffer B
Flow rate: 136 cm/hr (1.0 mL/min)
Detection: UV @ 280 nm
Sample: mAb in mouse ascites (dilution, ×5)
Load volume: 100 µL

Code #DescriptionLiterature Type
IM02 Instruction Manual for TOYOPEARL and TSKgel PW-type ResinsInstruction Manual
PCAT02 TOYOPEARL, TSKgel and Ca⁺⁺Pure-HA Bulk Resin 2019 Product Guide -- Ion ExchangeProduct Catalog
RIS0021 TOYOPEARL DEAE-650Resin Information Sheet
P/NDescriptionPrice ($) 
OC41MDAEX-96Resin Seeker TOYOPEARL AIEX 96-well plate kit384