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TOYOPEARL GigaCap CM-650M

TOYOPEARL GigaCap CM-650M resin is a high capacity cation exchange resin optimized for the high throughput purification of IgG’s.  The resins are composed of a base material of hydroxylated methacrylic polymer beads that have been chemically modified to provide a higher number of cationic binding sites and functionalized with carboxymethyl (CM) weak cation exchange groups.

TOYOPEARL GigaCap CM-650M resin exhibits different selectivity than the TOYOPEARL GigaCap S-650 resin. This difference, combined with its unusually high capacity for a weak cation exchange resin, may be exploited for either mAb or traditional protein purifications. TOYOPEARL GigaCap CM-650 resin is available in one particle size, 75 µm, which corresponds to the M-grade designation for TOYOPEARL resins.

TOYOPEARL GigaCap CM-650M exhibits typical dynamic binding capacities of 100 g/L for human IgG, maintains high capacity at increased linear velocities, has good pressure-flow characteristics, and will withstand back pressures up to 0.3 MPa.  

TOYOPEARL GigaCap CM-650M media has excellent binding and elution kinetics, which results in a more concentrated protein fraction collected during the elution step. TOYOPEARL GigaCap CM-650M media also has excellent caustic stability in 0.5 mol/L NaOH. 

 

 
P/NDescriptionResin VolumeParticle Size (µm)   Price
($)
 
21946TOYOPEARL GigaCap CM-650M100 mL75  457
21947TOYOPEARL GigaCap CM-650M250 mL75  851
21948TOYOPEARL GigaCap CM-650M1 L75  -
21949TOYOPEARL GigaCap CM-650M5 L75  -
21950TOYOPEARL GigaCap CM-650M50 L75  -
       

Product Attributes

Base bead: HW-65
Pore size (mean): 100 nm
Particle size (mean): 75 µm
Ligand type: weak cation
Ligand pKa: 3.6
DBC: > 110 g/L
Pressure rating: 0.3 MPa
Shipping buffer: 20% ethanol
pH stability: 3-13
Shelf life (estimated): 10 years

Structure:

IEX_GigaCap-CM-650M_Structure.png

 

           

Elution Pool Volume Comparison

IEX_GigaCap-CM-650M-Fig13.png

Resin: TOYOPEARL GigaCap CM-650M
Column size: 6.0 mm ID × 4 cm 
Mobile phase: Buffer A: 50 mmol/L sodium acetate buffer, pH 4.7
Buffer B: 50 mmol/L sodium acetate buffer, pH 4.7 + 0.5 mol/L NaCl
Flow rate: 212 cm/hr (1.0 mL/min)
Detection: UV @ 280 nm
Sample: polyclonal human IgG (1.0 mg/mL)



Pressure-Flow Comparison

IEX_GigaCap-CM-650M-Fig18.png

Resins: TOYOPEARL GigaCap CM-650M
TOYOPEARL GigaCap S-650M
CM Sepharose FF
Column size: 22 mm ID × 20 cm
Mobile phase: H2O
Detection: pressure (MPa)

Selectivity

IEX_GigaCap-CM-650M-Fig17.png

Resins: TOYOPEARL GigaCap CM-650M
TOYOPEARL GigaCap S-650M
Column size: 6.0 mm ID × 4 cm
Mobile phase: Buffer A: 20 mmol/L phosphate, pH 7.0
Buffer B: 20 mmol/L phosphate + 1.0 mol/L NaCl, pH 7.0
Gradient: 60 min linear gradient from buffer A to buffer B
Flow rate: 212 cm/hr (1.0 mL/min)
Detection: UV @ 280 nm
Injection vol.: 25 µL
Samples: (A) 1. ribonuclease A, 5.0 g/L
2. cytochrome C, 1.9 g/L
3. lysozyme, 3.8 g/L
(B) 1. trypsinogen, 3.8 g/L
2. ribonuclease A, 5.0 g/L
3. lysozyme, 3.8 g/L

Code #DescriptionLiterature Type
AN069 TOYOPEARL GigaCap Cation Exchange Resins for Aggregate Removal from Monoclonal Antibodies Application Note
BR04 TOYOPEARL GigaCap Ion Exchange ResinsBrochure
BR06 TOYOPEARL GigaCap Resins for Reducing Downstream WFI ConsumptionBrochure
IM02 Instruction Manual for TOYOPEARL and TSKgel PW-type ResinsInstruction Manual
PCAT02 TOYOPEARL, TSKgel and Ca⁺⁺Pure-HA Bulk Resin 2019 Product Guide -- Ion ExchangeProduct Catalog
PD16 Stability of TOYOPEARL Ion Exchange and Hydrophobic Interaction Chromatography Resins Stored in 2% Benzyl AlcoholPerformance Data
PO21 TOYOPEARL GigaCap CM-650M ResinProduct Overview
RIS0022 TOYOPEARL GigaCap-CM-650MResin Information Sheet
TP145 Purification of Monoclonal Antibodies Using Cation Exchange TOYOPEARL GigaCap ResinsTechnical Presentation
TP162 Characterization of a Novel High-Capacity Weak Cation Exchange ResinTechnical Presentation
P/NDescriptionPrice ($) 
OC41MDCEX-96Resin Seeker TOYOPEARL CIEX 96-well plate kit352
OC41MDGCCM-650MResin Seeker TOYOPEARL GigaCap CM-650M 96-well plate kit263
45227SkillPak CEX 1 mL column library304
45211SkillPak TOYOPEARL GigaCap CM-650M 1 mL columns (qty. 5)245
45247SkillPak TOYOPEARL GigaCap CM-650M 5 mL column204
43220TOYOPEARL CIEXPAK380
21951ToyoScreen GigaCap CM-650M, 1 mL (qty. 6)160
21952ToyoScreen GigaCap CM-650M, 5 mL (qty. 6)331
21394ToyoScreen IEC Cation Mix Pack, 1 mL (qty. 5)155
21395ToyoScreen IEC Cation Mix Pack, 5 mL (qty. 5)324
21396ToyoScreen IEC Mix Pack, 1 mL (qty. 5)155
21397ToyoScreen IEC Mix Pack, 5 mL (qty. 5)324
45005ToyoScreen RoboColumns GigaCap CM-650M, 200 µL216
45006ToyoScreen RoboColumns GigaCap CM-650M, 600 µL253