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TOYOPEARL SP-550C

TOYOPEARL SP-550C media is a strong cation exchanger for biomolecule purification. The resin is composed of a base material of hydroxylated methacrylic polymer beads that have been functionalized with sulfopropyl (S) strong cation exchange groups.  TOYOPEARL SP-550C resins have high chemical and mechanical stability, allowing cleaning-in-place (CIP) and very high flow rates.

TOYOPEARL SP-550C is currently available in only one particle size, 100 µm, and corresponds to the C -grade assigned to resins with this particle size. TOYOPEARL SP-550C is designed to be used for the high throughput capture of product from biological feedstock.

TOYOPEARL SP-550C exhibits typical dynamic binding capacities for lysozyme of 100 g/L.  TOYOPEARL SP-550C maintains capacity at increased linear velocities, has good pressure-flow characteristics, and will withstand back pressures up to 0.3 MPa.  

 

 
P/NDescriptionResin VolumeParticle Size (µm)   Price
($)
 
43272TOYOPEARL SP-550C100 mL100  210
14028TOYOPEARL SP-550C250 mL100  474
14705TOYOPEARL SP-550C1 L100  -
14029TOYOPEARL SP-550C5 L100  -
18366TOYOPEARL SP-550C50 L100  -
          

Product Attributes

Base bead: HW-55
Pore size (mean): 50 nm
Particle size (mean): 100 µm
Ligand type: strong cation
Ligand pKa: 1.2
DBC: 80 - 120 g/L
Pressure rating: 0.3 MPa
Shipping buffer: 20% ethanol
pH stability: 2-13
Shelf life: 10 years

Structure:

IEX_SP-550C_Structure.png

 

           

Dynamic Binding Capacity Comparison

Influence of pore size on lysozyme DBC 

IEX_SP-550_-650_CM650M_DBC.png


Resins: TOYOPEARL SP-650M
TOYOPEARL CM-650M
TOYOPEARL SP-550C
Mobile phase: 50 mmol/L phosphate buffer, pH 6.0
Flow rate: 100 cm/hr
Sample: lysozyme, 1 mg/mL

Code #DescriptionLiterature Type
IM02 Instruction Manual for TOYOPEARL and TSKgel PW-type ResinsInstruction Manual
RIS0027 TOYOPEARL SP-550CResin Information Sheet
P/NDescriptionPrice ($) 
OC41MDCEX-96Resin Seeker TOYOPEARL CIEX 96-well plate kit352
43220TOYOPEARL CIEXPAK380
21394ToyoScreen IEC Cation Mix Pack, 1 mL (qty. 5)155
21395ToyoScreen IEC Cation Mix Pack, 5 mL (qty. 5)324
21396ToyoScreen IEC Mix Pack, 1 mL (qty. 5)155
21397ToyoScreen IEC Mix Pack, 5 mL (qty. 5)324
21370ToyoScreen SP-550C, 1 mL (qty. 6)155
21371ToyoScreen SP-550C, 5 mL (qty. 6)324