Products

Process Media

TOYOPEARL SP-650M

TOYOPEARL SP-650M media is a strong cation exchanger for biomolecule purification. The resin is composed of a base material of hydroxylated methacrylic polymer beads that has been functionalized with sulfopropyl (S) strong cation exchange groups.  TOYOPEARL SP-650M resin has a high degree of chemical and mechanical stability, allowing cleaning-in-place (CIP) and very high flow rates. TOYOPEARL SP-650M resin has a 100 nm pore size and a 65 µm bead size.

TOYOPEARL SP-650M resin is best used for high throughput intermediate purification and polishing process steps. If greater resolution is needed, the smaller particle TOYOPEARL SP-650S or TSKgel SP-5PW resins may be used. As both the TOYOPEARL SP-650 and TSKgel SP-5PW resins use the same ligand and backbone chemistry, the selectivity between the two resin types will be unchanged.

TOYOPEARL SP-650M has a dynamic binding capacity for lysozyme of 50 g/L. TOYOPEARL SP-650M resin maintains capacity at increased linear velocities, has good pressure-flow characteristics, and will withstand back pressures up to 0.3 MPa.  

 

 
P/NDescriptionResin VolumeParticle Size (µm)   Price
($)
 
43202TOYOPEARL SP-650M100 mL65  208
07997TOYOPEARL SP-650M250 mL65  469
14699TOYOPEARL SP-650M1 L65  -
07998TOYOPEARL SP-650M5 L65  -
18369TOYOPEARL SP-650M50 L65  -
            

Product Attributes

Base bead: HW-65
Pore size (mean): 100 nm
Particle size (mean): 65 µm
Ligand type: strong cation
Ligand pKa: 1.2
DBC: 40 - 60 g/L
Pressure rating: 0.3 MPa
Shipping buffer: 20% ethanol
pH stability: 2-13
Shelf life: 10 years

Structure:

IEX_SP-650C-M-S_Structure.png

 
 
 

           

PEGylated Proteins

Breakthrough curves of mono-PEGylated lysozyme

IEX_SP-650M-Fig35.png



Resins: A. TOYOPEARL SP-650M
B. TOYOPEARL CM-650M
C. TOYOPEARL SP-550C
D. TOYOPEARL GigaCap CM-650M
E. TOYOPEARL GigaCap S-650M
Column size: 6 mm ID × 4 cm
Mobile phase: Buffer A: 20 mmol/L phosphate buffer, pH 7.0
Buffer B: 20 mmol/L phosphate buffer, pH 7.0 + 0.5 mol/L NaCl
Flow rate: 212 cm/hr (1.0 mL/min)
Detection: UV @ 280 nm
Sample: mono-PEGylated lysozyme, 1.0 g/L
(PEG MW = 5 kDa)

For additional applications, please see our applications database.

 
           

Dynamic Binding Capacity Comparison

Influence of pore size on lysozyme DBC 

IEX_SP-550_-650_CM650M_DBC.png


Resins: TOYOPEARL SP-650M
TOYOPEARL CM-650M
TOYOPEARL SP-550C
Mobile phase: 50 mmol/L phosphate buffer, pH 6.0
Flow rate: 100 cm/hr
Sample: lysozyme, 1 mg/mL

Code #DescriptionLiterature Type
AN024 Preserving Chromatographic Selectivity While Improving Your Process PurificationApplication Note
IM02 Instruction Manual for TOYOPEARL and TSKgel PW-type ResinsInstruction Manual
PD16 Stability of TOYOPEARL Ion Exchange and Hydrophobic Interaction Chromatography Resins Stored in 2% Benzyl AlcoholPerformance Data
RIS0028 TOYOPEARL SP-650Resin Information Sheet
P/NDescriptionPrice ($) 
45110MiniChrom TOYOPEARL SP-650M220
21394ToyoScreen IEC Cation Mix Pack, 1 mL (qty. 5)155
21395ToyoScreen IEC Cation Mix Pack, 5 mL (qty. 5)324
21368ToyoScreen SP-650M, 1 mL (qty. 6)155
21369ToyoScreen SP-650M, 5 mL (qty. 6)324