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TOYOPEARL SP-650C

TOYOPEARL SP-650C resin is a strong cation exchanger for biomolecule purification. The resin is composed of a base material of hydroxylated methacrylic polymer beads that have been functionalized with sulfopropyl (S) strong cation exchange groups.  TOYOPEARL SP-650C resin has a high degree of chemical and mechanical stability, allowing cleaning-in-place (CIP) and very high flow rates. TOYOPEARL SP-650C resin has a 100 nm pore size and a 100 µm bead size.

TOYOPEARL SP-650C resin can be used for high throughput capture, intermediate purification, and polishing process steps. If greater resolution is needed, the smaller particle TSKgel SP-5PW resins may be used. As both the TOYOPEARL SP-650C and TSKgel SP-5PW resins use the same ligand and backbone chemistry, the selectivity between the two resin types will be unchanged.

TOYOPEARL SP-650C resin has a dynamic binding capacity for lysozyme of 45 g/L. TOYOPEARL SP-650C resin maintains capacity at increased linear velocities, has good pressure-flow characteristics, and will withstand back pressures up to 0.3 MPa.  

 

 
P/NDescriptionResin VolumeParticle Size (µm)   Price
($)
 
07994TOYOPEARL SP-650C250 mL100  458
14700TOYOPEARL SP-650C1 L100  -
07995TOYOPEARL SP-650C5 L100  -
           

Product Attributes

Base bead: HW-65
Pore size (mean): 100 nm
Particle size (mean): 100 µm
Ligand type: strong cation
Ligand pKa: 1.2
DBC: 35 - 55 g/L
Pressure rating: 0.3 MPa
Shipping buffer: 20% ethanol
pH stability: 2-13
Shelf life: 10 years

Structure:

IEX_SP-650C-M-S_Structure.png

 
 

Code #DescriptionLiterature Type
AN024 Preserving Chromatographic Selectivity While Improving Your Process PurificationApplication Note
IM02 Instruction Manual for TOYOPEARL and TSKgel PW-type ResinsInstruction Manual
RIS0028 TOYOPEARL SP-650Resin Information Sheet