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TOYOPEARL Ether-650S

TOYOPEARL Ether-650S is a hydrophobic interaction chromatography (HIC) resin for biomolecule purification. The resin is composed of a base material of hydroxylated methacrylic polymer beads that have been functionalized with an Ether ligand group.  TOYOPEARL Ether-650S resin has a high degree of chemical and mechanical stability, allowing cleaning-in-place (CIP) and very high flow rates. TOYOPEARL Ether-650S resin has a 100 nm pore size and a 35 µm bead size and is the least hydrophobic of all TOYOPEARL HIC resins.  

TOYOPEARL Ether-650S resin can be used for high resolution intermediate purification and polishing process steps. If greater resolution is needed, the smaller particle TSKgel Ether-5PW resins may be used. If lower resolution is required, the TOYOPEARL Ether-650M may be used. As both the TOYOPEARL Ether-650 and TSKgel Ether-5PW resins use the same ligand and backbone chemistry, the selectivity between the two resin types will be unchanged.

TOYOPEARL Ether-650S resin has a dynamic binding capacity for lysozyme of 10 to 30 g/L. TOYOPEARL Ether-650S resin maintains capacity at increased linear velocities, has good pressure-flow characteristics, and will withstand back pressures up to 0.3 MPa.

 

 
P/NDescriptionResin VolumeParticle Size (µm)   Price
($)
 
43151TOYOPEARL Ether-650S25 mL35  232
16172TOYOPEARL Ether-650S100 mL35  725
16174TOYOPEARL Ether-650S1 L35  -
16176TOYOPEARL Ether-650S5 L35  -
       

Product Attributes

Base bead: HW-65
Pore size (mean): 100 nm
Particle size (mean): 35 µm 
Ligand type: Ether
DBC: 10 - 30 g/L 
Pressure rating: 0.3 MPa
Shipping buffer: 20% ethanol
pH stability: 1-13
Shelf life: 10 years

Structure:

HIC_Ether-650M-S_Structure.png

Hydrophobicity:

Ether < PPG < Phenyl < Butyl < Hexyl
(least to most)

 
 
Code #DescriptionLiterature Type
IM02 Instruction Manual for TOYOPEARL and TSKgel PW-type ResinsInstruction Manual
IM14 Instruction Manual -- Principles of Hydrophobic Interaction ChromatographyInstruction Manual
PCAT03 TOYOPEARL, TSKgel and Ca⁺⁺Pure-HA Bulk Resin 2019 Product Guide -- Hydrophobic InteractionProduct Catalog
RIS0013 TOYOPEARL Ether-650Resin Information Sheet
P/NDescriptionPrice ($) 
43150TOYOPEARL HICPAK HP580