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TOYOPEARL Ether-650M

TOYOPEARL Ether-650M is a hydrophobic interaction chromatography (HIC) resin for biomolecule purification. The resin is composed of a base material of hydroxylated methacrylic polymer beads that have been functionalized with an Ether ligand group.  TOYOPEARL Ether-650M resin has a high degree of chemical and mechanical stability, allowing cleaning-in-place (CIP) and very high flow rates. TOYOPEARL Ether-650M resin has a 100 nm pore size and a 65 µm bead size and is the least hydrophobic of the TOYOPEARL HIC resins.

TOYOPEARL Ether-650M resin can be used for high throughput capture, intermediate purification, and polishing process steps. If greater resolution is needed, the smaller particle TOYOPEARL Ether-650S or TSKgel Ether-5PW resins may be used. As both the TOYOPEARL Ether-650 and TSKgel Ether-5PW resins use the same ligand and backbone chemistry, the selectivity between the two resin types will be unchanged.

TOYOPEARL Ether-650M resin has a dynamic binding capacity for lysozyme of 10 to 30 g/L. TOYOPEARL Ether-650M resin maintains capacity at increased linear velocities, has good pressure-flow characteristics, and will withstand back pressures up to 0.3 MPa.

 

 
P/NDescriptionResin VolumeParticle Size (µm)   Price
($)
 
19805TOYOPEARL Ether-650M25 mL65  170
16173TOYOPEARL Ether-650M100 mL65  531
16175TOYOPEARL Ether-650M1 L65  -
16177TOYOPEARL Ether-650M5 L65  -
      

Product Attributes

Base bead: HW-65
Pore size (mean): 100 nm
Particle size (mean): 65 µm 
Ligand type: Ether
DBC: 10 - 30 g/L 
Pressure rating: 0.3 MPa
Shipping buffer: 20% ethanol
pH stability: 1-13
Shelf life: 10 years

Structure:

HIC_Ether-650M-S_Structure.png

Hydrophobicity:

Ether < PPG < Phenyl < Butyl < Hexyl
(least to most)

 
    

Purification of Monoclonal Antibodies


HIC_Ether-650M_figure_rev.png

Resins: A. TSKgel Ether-5PW (prepacked HPLC column)
B. TOYOPEARL Ether-650M
Column size: 7.5 mm ID × 7.5 cm
Mobile phase: Buffer A: 1.5 mol/L (NH4)2SO4+ 0.1 mol/L phosphate, pH 7.0
Buffer B: 0.1 mol/L phosphate, pH 7.0
Gradient: 60 min linear gradient from buffer A to B
Flow rate: 136 cm/hr (1.0 mL/min)
Detection: UV @ 280 nm
Temperature: ambient
Samples: A: 1.5 mg/100 µL anti-chicken 14 kDa lectin
B: 0.76 mg/50 µL diluted ascites fluid
Load volume: 150µL

For additional applications, please see our applications database.


           

Dynamic Binding Capacity Comparision for HIC Resins

Lysozyme

HIC_Ether-650M_PPG-600M_SuperButyl-550C_Hexyl-650_Fig6.png

Resins: binding capacity
(10% breakthrough)
TOYOPEARL Ether 650M 12.5 g/L
TOYOPEARL Phenyl 650M
27.5 g/L
TOYOPEARL Butyl 650M
32.2 g/L
TOYOPEARL Hexyl 650C
33.2 g/L
TOYOPEARL PPG 600M
44.2 g/L
TOYOPEARL SuperButyl 550C
54.3 g/L

 

Resins: A. TOYOPEARL Ether-650M
B. TOYOPEARL Phenyl-650M
C. TOYOPEARL Butyl-650M
D. TOYOPEARL Hexyl-650C
E. TOYOPEARL PPG-600M
F. TOYOPEARL SuperButyl-550C
Column size: 7.8 cm ID × 20 cm
Mobile phase: 1.8 mol/L sodium sulfate +
0.1 mol/L phosphate, pH 7.0
Flow rate: 100 cm/hr (0.8 mL/min)
Detection: UV @ 280 nm
Temperature: ambient
Sample: 1 g/L lysozyme
Sample load: as indicated in figure

Code #DescriptionLiterature Type
IM02 Instruction Manual for TOYOPEARL and TSKgel PW-type ResinsInstruction Manual
IM14 Instruction Manual -- Principles of Hydrophobic Interaction ChromatographyInstruction Manual
PCAT03 TOYOPEARL, TSKgel and Ca⁺⁺Pure-HA Bulk Resin 2019 Product Guide -- Hydrophobic InteractionProduct Catalog
PD05 Optimizing Pore Size to Improve Throughput in Downstream Purification of Biological Therapeutic ProductsPerformance Data
RIS0013 TOYOPEARL Ether-650Resin Information Sheet
P/NDescriptionPrice ($) 
OC41MDHIC-96Resin Seeker TOYOPEARL HIC 96-well plate kit449
19806TOYOPEARL HICPAK 323
21372ToyoScreen Ether-650M, 1 mL (qty. 6)202
21373ToyoScreen Ether-650M, 5 mL (qty. 6)420