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TOYOPEARL Phenyl-600M

TOYOPEARL Phenyl-600M is based on a 75 nm (750 Å) pore size, 65 µm polymethacrylate base material bonded with phenyl groups. Its pore size has been optimized for the purification of monoclonal antibodies. It has an intermediate hydrophobicity, which is slightly higher than the one of the 100 nm pore size TOYOPEARL Phenyl-650.

TOYOPEARL Phenyl-600M is typically used for intermediate purification or polishing steps. It is a very high recovery and high dynamic capacity resin for both large and small proteins. When used as the polishing step up to an extra 5% yield may be seen compared to other Phenyl resins.

 

 
P/NDescriptionResin VolumeParticle Size (µm)   Price
($)
 
21887TOYOPEARL Phenyl-600M25 mL65  180
21888TOYOPEARL Phenyl-600M100 mL65  567
21889TOYOPEARL Phenyl-600M1 L65  -
21890TOYOPEARL Phenyl-600M5 L65  -
21891TOYOPEARL Phenyl-600M50 L65  -
       

Product Attributes

Base bead: HW-60
Pore size (mean): 75 nm
Particle size (mean): 65 µm 
Ligand type: Phenyl
DBC: 45 - 65 g/L 
Pressure rating: 0.3 MPa
Shipping buffer: 20% ethanol
pH stability: 1-13
Shelf life: 10 years

Structure:

HIC_Phenyl-600M_Structure.png

Hydrophobicity:

Ether < PPG < Phenyl < Butyl <  Hexyl
(least to most)

 
 
            

Dynamic Binding Capacity of Polyclonal IgG on Various HIC Resins


HIC_Phenyl-600M_Fig4.png


Resins: A. TOYOPEARL PPG-600M
B. TOYOPEARL Phenyl-650M
C. TOYOPEARL Butyl-650M

D. Competitor Phenyl Agarose
E. TOYOPEARL Phenyl-600M
Column size: 7.8 mm ID × 20 cm
Mobile phase: 0.1 mol/L sodium phosphate, pH 7.0 + 0.8 mol/L (NH4)2SO4
Flow rate: 300 cm/hr (2.4 mL/min)
Detection: UV @ 280 nm
Temperature: 25 °C
Sample: 1.0 g/L polyclonal IgG

DBC was calculated at 10% breakthrough

Selectivity Comparison of Phenyl-type Resins


HIC_Phenyl-600M_650M-Fig5.png

Resins: A. TOYOPEARL Phenyl-600M
B. TOYOPEARL Phenyl-650M
C. Competitor Phenyl Agarose
Column size: 7.5 mm ID × 7.5 cm
Mobile phase: Buffer A: 1.8 mol/L (NH4)2SO4+ 0.1 mol/L sodium phosphate, pH 7.0
Buffer B: 0.1 mol/L sodium phosphate, pH 7.0
Gradient: 60 min linear gradient from buffer A to B
Flow rate: 136 cm/hr (1.0 mL/min)
Detection: UV @ 280 nm
Temperature: ambient
Sample: 1.0 g/L of: 1. ribonuclease A, 2. lysozyme
Load volume: 100 µL

 

Code #DescriptionLiterature Type
AN059 Hydrophobic Interaction Chromatography: Effects of Mixed Electrolytes on Protein SeparationsApplication Note
IM02 Instruction Manual for TOYOPEARL and TSKgel PW-type ResinsInstruction Manual
IM14 Instruction Manual -- Principles of Hydrophobic Interaction ChromatographyInstruction Manual
PCAT03 TOYOPEARL, TSKgel and Ca⁺⁺Pure-HA Bulk Resin 2019 Product Guide -- Hydrophobic InteractionProduct Catalog
PO18 TOYOPEARL Phenyl-600M for high capacity Hydrophobic Interaction Chromatography and high recovery of large and small proteinsProduct Overview
RIS0045 TOYOPEARL Phenyl-600MResin Information Sheet
TP124 Novel TOYOPEARL Hydrophobic Interaction Chromatographic Resin for Antibody PurificationTechnical Presentation
P/NDescriptionPrice ($) 
OC41MDHIC-96Resin Seeker TOYOPEARL HIC 96-well plate kit449
45234SkillPak HIC 1 mL column library245
45238SkillPak HIC 1 mL column library294
45236SkillPak HIC 1 mL column library294
45216SkillPak TOYOPEARL Phenyl-600M 1 mL columns (qty. 5)245
45252SkillPak TOYOPEARL Phenyl-600M 5 mL column204
21398ToyoScreen HIC Mix Pack, 1 mL (qty. 5)202
21399ToyoScreen HIC Mix Pack, 5 mL (qty. 5)420
21892ToyoScreen Phenyl-600M, 1 mL (qty. 6)202
21893ToyoScreen Phenyl-600M, 5 mL (qty. 6)420
45031ToyoScreen RoboColumns Phenyl-600M, 200 µL233
45032ToyoScreen RoboColumns Phenyl-600M, 600 µL307