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TOYOPEARL Butyl-650C

TOYOPEARL Butyl-650C is a hydrophobic interaction chromatography (HIC) resin for biomolecule purification. The resin is composed of a base material of hydroxylated methacrylic polymer beads that have been functionalized with a Butyl ligand group.  TOYOPEARL Butyl-650C resin has a high degree of chemical and mechanical stability, allowing cleaning-in-place (CIP) and very high flow rates. TOYOPEARL Butyl-650C resin has a 100 nm pore size and a 100 µm bead size and is the second most hydrophobic of the TOYOPEARL HIC resins.

TOYOPEARL Butyl-650C resin can be used for high throughput capture, intermediate purification, and polishing process steps. The primary applications for this resin are for the separation of proteins, their isoforms, and aggregate removal. If greater resolution is needed, the smaller particle TOYOPEARL Butyl-650M or TOYOPEARL Butyl-650S resins may be used.

TOYOPEARL Butyl-650C resin has a dynamic binding capacity for lysozyme of 30 to 50 g/L. TOYOPEARL Butyl-650C resin maintains capacity at increased linear velocities, has good pressure-flow characteristics, and will withstand back pressures up to 0.3 MPa. 

 

 
P/NDescriptionResin VolumeParticle Size (µm)   Price
($)
 
43127TOYOPEARL Butyl-650C25 mL100  137
07478TOYOPEARL Butyl-650C100 mL100  412
14703TOYOPEARL Butyl-650C1 L100  -
07977TOYOPEARL Butyl-650C5 L100  -
22826TOYOPEARL Butyl-650C50 L100  -
     

Product Attributes

Base bead: HW-65
Pore size (mean): 100 nm
Particle size (mean): 100 µm 
Ligand type: Butyl
DBC: 30 - 50 g/L 
Pressure rating: 0.3 MPa
Shipping buffer: 20% ethanol
pH stability: 1-13
Shelf life: 10 years

Structure:

HIC-Butyl-650_Structure_rev.png

Hydrophobicity:

Ether < PPG < Phenyl < Butyl < Hexyl
(least to most)

 
Code #DescriptionLiterature Type
IM02 Instruction Manual for TOYOPEARL and TSKgel PW-type ResinsInstruction Manual
IM14 Instruction Manual -- Principles of Hydrophobic Interaction ChromatographyInstruction Manual
PCAT03 TOYOPEARL, TSKgel and Ca⁺⁺Pure-HA Bulk Resin 2019 Product Guide -- Hydrophobic InteractionProduct Catalog
RIS0012 TOYOPEARL Butyl-650Resin Information Sheet
P/NDescriptionPrice ($) 
43125TOYOPEARL HICPAK-C492