Process Media


TOYOPEARL Butyl-600M is based on a 75 nm (750 Å) pore size polymethacrylate base material bonded with C4 groups. It has the second highest hydrophobicity of the TOYOPEARL HIC resins. Its pore size has been optimized for the purification of monoclonal antibodies and is between that of TOYOPEARL Butyl-650 and TOYOPEARL SuperButyl-550 media. TOYOPEARL Butyl-600M is typically used for intermediate purification or polishing steps. 


P/NDescriptionResin VolumeParticle Size (µm)   Price
21448TOYOPEARL Butyl-600M25 mL65  186
21449TOYOPEARL Butyl-600M100 mL65  585
21450TOYOPEARL Butyl-600M1 L65  -
21451TOYOPEARL Butyl-600M5 L65  -
21452TOYOPEARL Butyl-600M50 L65  -

Product Attributes

Base bead: HW-60
Pore size (mean): 75 nm
Particle size (mean): 65 µm 
Ligand type: Butyl
DBC: 40 - 60 g/L 
Pressure rating: 0.3 MPa
Shipping buffer: 20% ethanol
pH stability: 1-13
Shelf life: 10 years




Ether < PPG < Phenyl < Butyl < Hexyl
(least to most)


Selectivity Comparision of HIC -600M Resins


B. TOYOPEARL Phenyl-600M
Column size: 7.5 mm ID × 7.5 cm
Mobile phase: Buffer A: 1.8 mol/L (NH4)2SO+ 0.1 mol/L sodium phosphate, pH 7.0
Buffer B: 0.1 mol/L sodium phosphate, pH 7.0
Gradient: 60 min linear gradient from buffer A to B
Flow rate: 136 cm/hr (1.0 mL/min)
Detection: UV @ 280 nm
Temperature: ambient
Samples: 1 g/L of: 1. ribonuclease A, 2. lysozyme, 3. α-chymotrypsinogen
Load volume: 100 µL


Code #DescriptionLiterature Type
AN059 Hydrophobic Interaction Chromatography: Effects of Mixed Electrolytes on Protein SeparationsApplication Note
IM02 Instruction Manual for TOYOPEARL and TSKgel PW-type ResinsInstruction Manual
IM14 Instruction Manual -- Principles of Hydrophobic Interaction ChromatographyInstruction Manual
PCAT03 TOYOPEARL, TSKgel and Ca⁺⁺Pure-HA Bulk Resin 2019 Product Guide -- Hydrophobic InteractionProduct Catalog
PD05 Optimizing Pore Size to Improve Throughput in Downstream Purification of Biological Therapeutic ProductsPerformance Data
PD16 Stability of TOYOPEARL Ion Exchange and Hydrophobic Interaction Chromatography Resins Stored in 2% Benzyl AlcoholPerformance Data
PO05 TOYOPEARL Butyl-600M, for Hydrophobic Interaction Chromatography of monoclonal antibodies and hydrophilic proteinsProduct Overview
RIS0011 TOYOPEARL Butyl-600MResin Information Sheet
TP104 Maximizing IgG binding capacity of hydrophobic interaction chromatography resins using pore size optimizationTechnical Presentation
TP113 Purification of monoclonal antibodies using new pore size optimized HIC resinsTechnical Presentation
P/NDescriptionPrice ($) 
OC41MDHIC-96Resin Seeker TOYOPEARL HIC 96-well plate kit493
45234SkillPak HIC 1 mL column library254
45236SkillPak HIC 1 mL column library305
45214SkillPak TOYOPEARL Butyl-600M 1 mL columns (qty. 5)253
45250SkillPak TOYOPEARL Butyl-600M 5 mL column210
21494ToyoScreen Butyl-600M, 1 mL (qty. 6)209
21495ToyoScreen Butyl-600M, 5 mL (qty. 6)433
21398ToyoScreen HIC Mix Pack, 1 mL (qty. 5)209
21399ToyoScreen HIC Mix Pack, 5 mL (qty. 5)433
45033ToyoScreen RoboColumns Butyl-600M, 200 µL260
45034ToyoScreen RoboColumns Butyl-600M, 600 µL340