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TOYOPEARL Hexyl-650C

TOYOPEARL Hexyl-650C is based on a 100 nm (1000 Å) pore size, 100 µm polymethacrylate base material bonded with C6 groups. This resin has the highest hydrophobicity of the TOYOPEARL HIC resins. It is used for very hydrophilic proteins or where a low salt elution environment is required. It is useful as an early capture step or for intermediate purification.

 

 
P/NDescriptionResin VolumeParticle Size (µm)   Price
($)
 
44465TOYOPEARL Hexyl-650C25 mL100  173
19026TOYOPEARL Hexyl-650C100 mL100  541
19027TOYOPEARL Hexyl-650C1 L100  -
19028TOYOPEARL Hexyl-650C5 L100  -
21973TOYOPEARL Hexyl-650C50 L100  -
       

Product Attributes

Base bead: HW-65
Pore size (mean): 100 nm
Particle size (mean): 100 µm 
Ligand type: Hexyl
DBC: 30 - 50 g/L 
Pressure rating: 0.3 MPa
Shipping buffer: 20% ethanol
pH stability: 1-13
Shelf life: 10 years

Structure:

HIC_Hexyl-650C_Structure.png

Hydrophobicity:

Ether < PPG < Phenyl < Butyl < Hexyl
(least to most)

 
 
    

Plasmid DNA Purification


HIC-Hexyl-650C-Fig13_rev.png


Resin: TOYOPEARL Hexyl-650C
Column size: 1.0 cm ID × 30 cm (23.6 mL)
Mobile phase: Buffer A: 3.0 mol/L ammonium sulfate, 10 mmol/L Tris, 1 mmol/L EDTA, pH 7.4
Buffer B: 10 mmol/L Tris, 1 mmol/L EDTA, pH 7.4
Gradient: 3.0 mol/L ammonium sulfate to 1.0 mol/L ammonium sulfate (6 CV)
Flow rate: 153 cm/hr (2.0 mL/min)
Detection: UV @ 254 nm
Samples: Plasmid DNA in 3.0 mol/L ammonium sulfate

For additional applications, please see our applications database.

           

Dynamic Binding Capacity Comparision for HIC Resins

Lysozyme

HIC_Ether-650M_PPG-600M_SuperButyl-550C_Hexyl-650_Fig6.png

Resins: binding capacity
(10% breakthrough)
TOYOPEARL Ether 650M 12.5 g/L
TOYOPEARL Phenyl 650M
27.5 g/L
TOYOPEARL Butyl 650M
32.2 g/L
TOYOPEARL Hexyl 650C
33.2 g/L
TOYOPEARL PPG 600M
44.2 g/L
TOYOPEARL SuperButyl 550C
54.3 g/L

 

Resins: A. TOYOPEARL Ether-650M
B. TOYOPEARL Phenyl-650M
C. TOYOPEARL Butyl-650M
D. TOYOPEARL Hexyl-650C
E. TOYOPEARL PPG-600M
F. TOYOPEARL SuperButyl-550C
Column size: 7.8 cm ID × 20 cm
Mobile phase: 1.8 mol/L sodium sulfate +
0.1 mol/L phosphate,pH 7.0
Flow rate: 100 cm/hr (0.8 mL/min)
Detection: UV @ 280 nm
Temperature: ambient
Sample: 1 g/L lysozyme
Sample load: as indicated in figure

Code #DescriptionLiterature Type
IM02 Instruction Manual for TOYOPEARL and TSKgel PW-type ResinsInstruction Manual
IM14 Instruction Manual -- Principles of Hydrophobic Interaction ChromatographyInstruction Manual
PCAT03 TOYOPEARL, TSKgel and Ca⁺⁺Pure-HA Bulk Resin 2019 Product Guide -- Hydrophobic InteractionProduct Catalog
PD05 Optimizing Pore Size to Improve Throughput in Downstream Purification of Biological Therapeutic ProductsPerformance Data
RIS0014 TOYOPEARL Hexyl-650CResin Information Sheet
P/NDescriptionPrice ($) 
OC41MDHIC-96Resin Seeker TOYOPEARL HIC 96-well plate kit449
43125TOYOPEARL HICPAK-C492
21378ToyoScreen Hexyl-650C, 1 mL (qty. 6)202
21379ToyoScreen Hexyl-650C, 5 mL (qty. 6)420
21398ToyoScreen HIC Mix Pack, 1 mL (qty. 5)202
21399ToyoScreen HIC Mix Pack, 5 mL (qty. 5)420