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TOYOPEARL AF-rProtein A-650F

TOYOPEARL AF-rProtein A-650F resin is an alkaline resistant protein A resin developed for the capture and purification of monoclonal antibodies.  The resins are composed of a base material of hydroxylated methacrylic polymer beads that have been chemically modified to provide multipoint attachment when functionalized with the protein A ligand.

TOYOPEARL AF-rProtein A-650F utilizes an alkaline resistant recombinant Protein A variant. This non-animal derived ligand is bound to the base bead, Toyopearl HW-65F, using multipoint attachment. This contributes to both the resin's alkaline resistance and its low ligand leakages in the range of 5-25ng/mg IgG. Good pressure-flow characteristics and kinetics contribute to maintaining good capacity, particularly at low residence times.

Toyopearl AF-rProtein A-650F maintains its capacity well after repeated CIP exposure to 0.1mol/L NaOH and has reasonable tolerance to CIP with 0.5mol/L NaOH. TOYOPEARL AF-rProtein A-650F also tolerates exposure to above ambient temperatures and if needed can be autoclaved.

TOYOPEARL AF-rProtein A-650F is available in one particle size, 45 µm, which corresponds to the F-grade designation for TOYOPEARL resins.

TOYOPEARL AF-rProtein A-650F exhibits typical dynamic binding capacities of ≥30 g/L for human IgG at 2 minutes residence time, maintains high capacity at increased linear velocities has good pressure-flow characteristics and will withstand back pressures up to 0.3 MPa.

NOW AVAILABLE - NEW PROTEIN A ELISA KIT optimized for TOYOPEARL AF-rProtein A HC-650F and TOYOPEARL AF-rProtein A-650F resins
Tosoh R40 and R28 Protein A, Mix-N-Go ELISA Kit from Cygnus Technologies, catalog # F910. (https://cygnustechnologies.com/product_detail/tosoh-r40-and-r28-protein-a-mix-n-go-elisa.html)

 

 
P/NDescriptionResin VolumeParticle Size (µm)   Price
($)
 
22803TOYOPEARL AF-rProtein A-650F10 mL45  807
22804TOYOPEARL AF-rProtein A-650F25 mL45  1,381
22805TOYOPEARL AF-rProtein A-650F100 mL45  2,758
22806TOYOPEARL AF-rProtein A-650F1 L45  -
22807TOYOPEARL AF-rProtein A-650F5 L45  -
22808TOYOPEARL AF-rProtein A-650F50 L45  -
        

Product Attributes

Pore size (mean): 100 nm
Particle size (mean): 45 µm
Pressure rating: 0.3 MPa
Shipping buffer: 20% ethanol
pH stability: 2-13
Shelf life (room temp.): 1.5 years (on going)

Structure:

AFC_rProteinA-650F_structure.png

 
      

CIP Stability

0.1 mol/L NaOH

ProA_AF-rProtein-A-HC-650F-Fig18.png

Resin: TOYOPEARL AF-rProtein A-650F
Column size: 4.6 mm ID × 10 cm
Mobile phase: Buffer A: 0.15 mol/L NaCl in 0.02 mol/L sodium phosphate buffer, pH 7.2
Buffer B: 0.10 mol/L citrate buffer, pH 3.0
Cycle volumes: 5 column volumes binding buffer
10 column volumes elution buffer
3 column volumes 0.1 mol/L NaOH, (16 min contact time)
5 column volumes H20

DBC was calculated at 10% breakthrough

0.5 mol/L NaOH

ProA_AF-rProtein-A-HC-650F-Fig19.png

Resin: TOYOPEARL AF-rProtein A-650F
Column size: 5.0 mm ID × 5 cm
Mobile phase: Buffer A: 0.15 mol/L NaCl in 0.02 mol/L sodium phosphate buffer, pH 7.2
Buffer B: 0.10 mol/L citrate buffer, pH 3.0
Cycle volumes: 5 column volumes binding buffer
10 column volumes elution buffer
8 column volumes 0.5 mol/L NaOH, (16 min contact time)
5 column volumes H20

DBC was calculated at 10% breakthrough


Temperature Stability

ProA_AF-rProtein-A-HC-650F-Fig20.png

Resin: TOYOPEARL AF-rProtein A-650F
Mobile phase: deionized H20
Autoclave settings: 120 °C, 1.2 atm, 15 min
Heating time: 30 min

TOYOPEARL AF-rProtein A-650F is stable at 35 °C for at least 3 years (data not shown).

Benzyl Alcohol Removal

ProA_AF-rProtein-A-650F-Fig23.png

Resin: TOYOPEARL AF-rProtein A-650F
Column size: 1.6 cm ID × 10 cm (20.1 mL)
Mobile phase: deionized H20
Flow rate: 800 cm/hr (26.8 mL/min)
Residence time: 45 sec

 

Code #DescriptionLiterature Type
AN056 Clearance of 2% Benzyl Alcohol from TOYOPEARL ResinsApplication Note
IM02 Instruction Manual for TOYOPEARL and TSKgel PW-type ResinsInstruction Manual
PCAT04 TOYOPEARL, TSKgel and Ca⁺⁺Pure-HA Bulk Resin 2019 Product Guide -- AffinityProduct Catalog
RIS0008 TOYOPEARL AF-Protein-A-650FResin Information Sheet
TP198 Characterization of a Newly Developed High Capacity, Alkaline Resistant, Recombinant Protein A ResinTechnical Presentation
P/NDescriptionPrice ($) 
22809ToyoScreen AF-rProtein A-650F, 1 mL (qty. 5)749
22810ToyoScreen AF-rProtein A-650F, 5 mL (qty. 1)693
22811ToyoScreen AF-rProtein A-650F, 5 mL (qty. 5) 2,242
45061ToyoScreen RoboColumns AF-rProtein A-650F, 200 µL317
45062ToyoScreen RoboColumns AF-rProtein A-650F, 600 µL1,107