Process Media


TOYOPEARL AF-Carboxy-650M is a reactive resin that requires activation for ligand attachment. It provides mild coupling to amino groups of proteins or low molecular weight ligands. The carbodiimide mediated coupling reaction produces an amide bond between ligand and support.


P/NDescriptionResin VolumeParticle Size (µm)   Price
43412TOYOPEARL AF-Carboxy-650M10 mL65  131
08006TOYOPEARL AF-Carboxy-650M25 mL65  267
08041TOYOPEARL AF-Carboxy-650M100 mL65  1,028
18827TOYOPEARL AF-Carboxy-650M1 L65  -
18828TOYOPEARL AF-Carboxy-650M5 L65  -

Product Attributes

Pore size (mean): 100 nm
Particle size (mean): 65 µm
Pressure rating: 0.3 MPa
Shipping buffer: 20% ethanol
pH stability: 2-13
Shelf life: 10 years




Coupling procedure


Representative coupling densities

Protein coupled g/L resin
soybean trypsin inhibitor 15
ovalbumin 0.8
bovine serum albumin 3.3
human IgG 11.7
cytochrome 7.5
lysozyme 17.5

coupling agent: carbodiimide
optimal pH: 4.5 - 6.0

Code #DescriptionLiterature Type
IM02 Instruction Manual for TOYOPEARL and TSKgel PW-type ResinsInstruction Manual
IM07 Instruction Manual -- TOYOPEARL AF-Carboxy-650MInstruction Manual
PCAT04 TOYOPEARL, TSKgel and Ca⁺⁺Pure-HA Bulk Resin 2019 Product Guide -- AffinityProduct Catalog
RIS0002 TOYOPEARL AF-Carboxy-650MResin Information Sheet
P/NDescriptionPrice ($)