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TOYOPEARL AF-Epoxy-650M

TOYOPEARL AF-Epoxy-650M is an activated resin with a high density of epoxy-functionality, packaged in dry form. It can be used to immobilize proteins or low molecular weight ligands. It is particularly useful when high densities of low molecular weight ligands must be attached. It is a highly versatile starting material for conversion to other chemically active functional groups and may be readily activated to hydrazide-bearing materials. TOYOPEARL AF-Epoxy-650M is particularly useful for immobilization of carbohydrates or glycoproteins.

 

 
P/NDescriptionResin VolumeParticle Size (µm)   Price
($)
 
43402TOYOPEARL AF-Epoxy-650M5 g65  174
08000TOYOPEARL AF-Epoxy-650M10 g65  306
08038TOYOPEARL AF-Epoxy-650M100 g65  2,011
18315TOYOPEARL AF-Epoxy-650M1 kg65  -
         

Product Attributes

Pore size (mean): 100 nm
Particle size (mean): 65 µm
Pressure rating: 0.3 MPa
Shipping condition: dry
Storage temperature: ambient


Structure:

AFC_AF-Epoxy-650M_Structure.png

 
 
 
   

Purification of Lectins

AFC_AF-Epoxy-650_Fig13_rev.png

 

Resin: GaINAc-Epoxy TOYOPEARL
Column size:  10 mm ID × 5 cm 
Mobile phase:  Buffer A: 10 mmol/L phosphate-buffered saline, pH 7.4
Buffer B: buffer A + 0.1 mol/L lactose 
Gradient:  equilibrate/load/wash 100% buffer A
isocratic elution 100% buffer B
Detection: UV @ 275 nm 
Sample: 4.0 mg impure Grifola frondosa lectin


 

For additional applications, please see our applications database.

 
        

Coupling procedure


AFC_AF-Epoxy_coupling.png

 

 

 
Code #DescriptionLiterature Type
IM02 Instruction Manual for TOYOPEARL and TSKgel PW-type ResinsInstruction Manual
IM09 Instruction Manual -- TOYOPEARL AF-Epoxy-650MInstruction Manual
PCAT04 TOYOPEARL, TSKgel and Ca⁺⁺Pure-HA Bulk Resin 2019 Product Guide -- AffinityProduct Catalog
RIS0004 TOYOPEARL AF-Epoxy-650MResin Information Sheet
P/NDescriptionPrice ($) 
43400TOYOPEARL AFFIPAK ACT326